Human IL-18BP protein (Recombinant) (C-hFc&His) (STJP000937)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human IL-18BP Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Thr31-Gly194) of human IL18BPa (Accession #NP_001034748.1) fused with an Fc, 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human IL-18BP-C-hFc&His protein was developed in hek293 cells using the region Thr31-Gly194. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its binding ability in a functional ELISA. Immobilized Human IL18BP at 2 Mu g/mL (100 Mu L/well) can bind IL18 with a linear range of 0.128-47.02 ng/mL. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | IL18BP |
| Gene ID | 10068 |
| Uniprot ID | I18BP_HUMAN |
| Immunogen | Recombinant Human IL-18BP Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Thr31-Gly194) of human IL18BPa (Accession #NP_001034748.1) fused with an Fc, 6×His tag at the C-terminus. |
| Immunogen Region | Thr31-Gly194 |
| Immunogen Sequence | TPVSQTTTAATASVRSTKDP CPSQPPVFPAAKQCPALEVT WPEVEVPLNGTLSLSCVACS RFPNFSILYWLGNGSFIEHL PGRLWEGSTSRERGSTGTQL CKALVLEQLTPALHSTNFSC VLVDPEQVVQRHVVLAQLWA GLRATLPPTQEALPSSHSSP QQQG |
Additional Info
| Tissue Specificity | Strongly expressed in heart, lung, placenta and spleen. |
| Post Translational Modifications | N- and O-glycosylated. O-glycosylated with core 1-like and core 2-like glycans. O-glycan heterogeneity at Ser-53: HexHexNAc (major) and Hex2HexNAc2 (minor). N-glycan heterogeneity at Asn-103: Hex5HexNAc4 (minor), dHex1Hex5HexNAc4 (major) and Hex6HexNAc5 (minor).N-glycan at Asn-147: dHex1Hex5HexNAc4. |
| Function | Isoform A binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response. |
| Protein Name | Interleukin-18-Binding ProteinIl-18bpTadekinig-Alfa |
| Database Links | Reactome: R-HSA-9008059Reactome: R-HSA-9012546 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Interleukin-18-Binding Protein proteinIl-18bp proteinTadekinig-Alfa proteinIL18BP protein |
Information sourced from Uniprot.org