Human IL-18 protein (Recombinant) (No tag) (STJP000779)
SPECIFICATIONS
HostE.coli
ConjugationUnconjugated
ImmunogenRecombinant Human IL-18 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Tyr37-Asp193) of human IL-18 (Accession #NP_001553.1).
General Information
| Short Description | Recombinant-Human IL-18-No tag protein was developed in e.coli using the region Tyr37-Asp193. For use in research applications. |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | 1.Measured by its binding ability in a functional ELISA. Immobilized recombinant Human IL18 at 5 Mu g/mL (100 Mu L/well) can bind recombinant Human IL18BPa with a linear range of 0.156-1.372 Mu g/mL.|2.Measured by its ability to induce IFN-gamma secr |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | IL18 |
| Gene ID | 3606 |
| Uniprot ID | IL18_HUMAN |
| Immunogen | Recombinant Human IL-18 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Tyr37-Asp193) of human IL-18 (Accession #NP_001553.1). |
| Immunogen Region | Tyr37-Asp193 |
| Immunogen Sequence | YFGKLESKLSVIRNLNDQVL FIDQGNRPLFEDMTDSDCRD NAPRTIFIISMYKDSQPRGM AVTISVKCEKISTLSCENKI ISFKEMNPPDNIKDTKSDII FFQRSVPGHDNKMQFESSSY EGYFLACEKERDLFKLILKK EDELGDRSIMFTVQNED |
Additional Info
| Tissue Specificity | Isoform 2: Expressed in ovarian carcinoma but undetectable in normal ovarian epithelial cells. Resistant to proteolytic activation by caspase-1 and -4. |
| Post Translational Modifications | The pro-IL-18 precursor is processed by CASP1, CASP4 or CASP5 to yield its mature, active form. The pro-IL-18 precursor features autoinhibitory interactions between the propeptide and the post-cleavage-site region, preventing recognition by the IL18R1 receptor. Processing by CASP1, CASP4 or CASP5 induces conformational changes to generate critical receptor-binding sites. The mature form is then secreted and released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. In contrast, cleavage by CASP3 inactivates IL18. |
| Function | Pro-inflammatory cytokine primarily involved in epithelial barrier repair, polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses. Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. |
| Protein Name | Interleukin-18Il-18IboctadekinInterferon Gamma-Inducing FactorIfn-Gamma-Inducing FactorInterleukin-1 GammaIl-1 Gamma |
| Database Links | Reactome: R-HSA-448706Reactome: R-HSA-5620971Reactome: R-HSA-6783783Reactome: R-HSA-6785807Reactome: R-HSA-9012546 Q14116-2Reactome: R-HSA-9660826 |
| Cellular Localisation | CytoplasmCytosolSecretedThe Precursor Is CytosolicIn Response To Inflammasome-Activating SignalsCleaved And SecretedMature Form Is Secreted And Released In The Extracellular Milieu By Passing Through The Gasdermin-D (Gsdmd) PoreIn ContrastThe Precursor Form Is Not ReleasedDue To The Presence Of An Acidic Region That Is Proteolytically Removed By Casp1Casp4 Or Casp5 During MaturationThe Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10 |
| Alternative Protein Names | Interleukin-18 proteinIl-18 proteinIboctadekin proteinInterferon Gamma-Inducing Factor proteinIfn-Gamma-Inducing Factor proteinInterleukin-1 Gamma proteinIl-1 Gamma proteinIL18 proteinIGIF proteinIL1F4 protein |
Information sourced from Uniprot.org