Human IGFBP4 (Insulin-like growth factor-binding protein 4) Sandwich ELISA Kit (STJE0002234)

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STJE0002234
$220.73 - $482.63
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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 1.88ng/mL
Detection Limit: 3.13~200ng/mL
Short Description : This IGFBP4 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: IGFBP4
Gene ID: 3487
Uniprot ID: IBP4_HUMAN
Specificity: This kit recognizes Human IGFBP-4 in samples. No significant cross-reactivity or interference between Human IGFBP-4 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Protein Name Insulin-Like Growth Factor-Binding Protein 4
Ibp-4
Igf-Binding Protein 4
Igfbp-4
Database Links Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Cellular Localisation Secreted
Alternative ELISA Names Insulin-Like Growth Factor-Binding Protein 4 ELISA kit
Ibp-4 ELISA kit
Igf-Binding Protein 4 ELISA kit
Igfbp-4 ELISA kit
IGFBP4 ELISA kit
IBP4 ELISA kit
Specificity This kit recognizes Human IGFBP-4 in samples. No significant cross-reactivity or interference between Human IGFBP-4 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 94-105 99
EDTA plasma (n=8) 85-97 92
Cell culture media (n=8) 97-109 102

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 10.62 31.89 73.95 10.52 29.18 74.97
Standard deviation 0.60 1.79 3.21 0.59 1.47 3.63
CV (%) 5.65 5.61 4.34 5.61 5.04 4.84

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