Human IGFBP4 (Insulin-like growth factor-binding protein 4) Sandwich ELISA Kit (STJE0002234)
SPECIFICATIONS
General Information
| Short Description | This IGFBP4 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids. |
| Applications | ELISA |
| Reactivity | Human |
| Sensitivity | 1.88ng/mL |
| Detection Limit | 3.13~200ng/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Colormetric |
Target Information
| Gene Symbol | IGFBP4 |
| Gene ID | 3487 |
| Uniprot ID | IBP4_HUMAN |
| Specificity | This kit recognizes Human IGFBP-4 in samples. No significant cross-reactivity or interference between Human IGFBP-4 and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Protein Name | Insulin-Like Growth Factor-Binding Protein 4Ibp-4Igf-Binding Protein 4Igfbp-4 |
| Database Links | Reactome: R-HSA-381426Reactome: R-HSA-8957275 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Insulin-Like Growth Factor-Binding Protein 4 ELISA kitIbp-4 ELISA kitIgf-Binding Protein 4 ELISA kitIgfbp-4 ELISA kitIGFBP4 ELISA kitIBP4 ELISA kit |
| Specificity | This kit recognizes Human IGFBP-4 in samples. No significant cross-reactivity or interference between Human IGFBP-4 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 94-105 | 99 |
| EDTA plasma (n=8) | 85-97 | 92 |
| Cell culture media (n=8) | 97-109 | 102 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 10.62 | 31.89 | 73.95 | 10.52 | 29.18 | 74.97 |
| Standard deviation | 0.60 | 1.79 | 3.21 | 0.59 | 1.47 | 3.63 |
| CV (%) | 5.65 | 5.61 | 4.34 | 5.61 | 5.04 | 4.84 |