| Host: |
CHO cells |
| Note: |
STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Human IGFBP-3-protein was developed from cho cells. For use in research applications. |
| Conjugation: |
Unconjugated |
| Formulation: |
Lyophilised from 0.2 Mu m filtered PBS solution. |
| Dilution Range: |
Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Storage Instruction: |
Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin: |
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Immunoreactivity: |
The ED (50) was determined by the ability toinhibit IGF-II induced proliferation of MCF-7 and was foundto be≤ 0.2 Mu g/ml in the presence of 15 ng/ml human IGF-II. NA |
| Gene Symbol: |
IGFBP3 |
| Gene ID: |
3486 |
| Uniprot ID: |
IBP3_HUMAN |
| Immunogen Region: |
Mature chain |
| Immunogen: |
Optimized DNA sequence encoding HumanIGFBP-3 mature chain fused to an N-terminal His tag was expressed in CHO Cells. NA |
| Immunogen Sequence: |
NA |
| Tissue Specificity | Expressed by most tissues. Present in plasma. |
| Post Translational Modifications | Phosphorylated by FAM20C in the extracellular medium. |
| Function | IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R. Inhibits the positive effect of humanin on insulin sensitivity. Promotes testicular germ cell apoptosis. |
| Protein Name | Insulin-Like Growth Factor-Binding Protein 3Ibp-3Igf-Binding Protein 3Igfbp-3 |
| Database Links | Reactome: R-HSA-381426Reactome: R-HSA-6803211Reactome: R-HSA-8957275 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Insulin-Like Growth Factor-Binding Protein 3 proteinIbp-3 proteinIgf-Binding Protein 3 proteinIgfbp-3 proteinIGFBP3 proteinIBP3 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
