Human IGFBP-3 protein (Recombinant) (STJP000459)
SPECIFICATIONS
HostCHO Cells
ConjugationUnconjugated
ImmunogenOptimized DNA sequence encoding HumanIGFBP-3 mature chain fused to an N-terminal His tag was expressed in CHO Cells. NA
General Information
| Short Description | Recombinant-Human IGFBP-3-protein was developed from cho cells. For use in research applications. |
| Host | CHO Cells |
| Note | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Formulation | Lyophilised from 0.2 Mu m filtered PBS solution. |
| Storage Instruction | Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Immunoreactivity | The ED (50) was determined by the ability toinhibit IGF-II induced proliferation of MCF-7 and was foundto be≤ 0.2 Mu g/ml in the presence of 15 ng/ml human IGF-II. NA |
Target Information
| Gene Symbol | IGFBP3 |
| Gene ID | 3486 |
| Uniprot ID | IBP3_HUMAN |
| Immunogen | Optimized DNA sequence encoding HumanIGFBP-3 mature chain fused to an N-terminal His tag was expressed in CHO Cells. NA |
| Immunogen Region | Mature chain |
| Immunogen Sequence | NA |
Additional Info
| Tissue Specificity | Expressed by most tissues. Present in plasma. |
| Post Translational Modifications | Phosphorylated by FAM20C in the extracellular medium. Phosphorylated by CK2.resulting in decreased nuclear localization. |
| Function | Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R. Inhibits the positive effect of humanin on insulin sensitivity. Promotes testicular germ cell apoptosis. Acts via LRP-1/alpha2M receptor, also known as TGF-beta type V receptor, to mediate cell growth inhibition independent of IGF1. Mechanistically, induces serine-specific dephosphorylation of IRS1 or IRS2 upon ligation to its receptor, leading to the inhibitory cascade. In the nucleus, interacts with transcription factors such as retinoid X receptor-alpha/RXRA to regulate transcriptional signaling and apoptosis. |
| Protein Name | Insulin-Like Growth Factor-Binding Protein 3Ibp-3Igf-Binding Protein 3Igfbp-3 |
| Database Links | Reactome: R-HSA-381426Reactome: R-HSA-6803211Reactome: R-HSA-8957275 |
| Cellular Localisation | SecretedNucleus |
| Alternative Protein Names | Insulin-Like Growth Factor-Binding Protein 3 proteinIbp-3 proteinIgf-Binding Protein 3 proteinIgfbp-3 proteinIGFBP3 proteinIBP3 protein |
Information sourced from Uniprot.org