Human ICOS/CD278 protein (Recombinant) (C-hFc&His) (STJP001393)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human ICOS/CD278 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Glu21-Phe141) of human ICOS/CD278 (Accession #NP_036224.1) fused with a Fc, 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human ICOS/CD278-C-hFc&His protein was developed in hek293 cells using the region Glu21-Phe141. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its binding ability in a functional ELISA. Immobilized human B7-H2/ICOSLG at 1 Mu g/mL (100 Mu L/well) can bind Human ICOS/CD278 with a linear range of 2-14.8 ng/mL. |
| Determination Method | <0.1 EU/Mu g |
Target Information
| Gene Symbol | ICOS |
| Gene ID | 29851 |
| Uniprot ID | ICOS_HUMAN |
| Immunogen | Recombinant Human ICOS/CD278 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Glu21-Phe141) of human ICOS/CD278 (Accession #NP_036224.1) fused with a Fc, 6×His tag at the C-terminus. |
| Immunogen Region | Glu21-Phe141 |
| Immunogen Sequence | EINGSANYEMFIFHNGGVQI LCKYPDIVQQFKMQLLKGGQ ILCDLTKTKGSGNTVSIKSL KFCHSQLSNNSVSFFLYNLD HSHANYYFCNLSIFDPPPFK VTLTGGYLHIYESQLCCQLK F |
Additional Info
| Post Translational Modifications | N-glycosylated. |
| Function | Stimulatory receptor expressed in activated or antigen-experienced T-cells that plays an important role in the immune response. Upon binding to its ligand ICOSL expressed on antigen presenting cells (APCs), delivers costimulatory signals that enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines including IL10, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Also acts as a costimulatory receptor critical for the differentiation of T follicular regulatory cells upon immune challenges such as viral infection. Mechanistically, potentiates TCR-induced calcium flux by augmenting PLCG1 activation and actin remodeling. In addition, activates PI3K signaling pathways independently of calcium flux. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes. |
| Protein Name | Inducible T-Cell CostimulatorActivation-Inducible Lymphocyte Immunomediatory MoleculeCd Antigen Cd278 |
| Database Links | Reactome: R-HSA-1257604 Q9Y6W8-1Reactome: R-HSA-2219530 Q9Y6W8-1Reactome: R-HSA-6811558 Q9Y6W8-1Reactome: R-HSA-9725371Reactome: R-HSA-9927354 Q9Y6W8-1 |
| Cellular Localisation | Isoform 1: Cell MembraneSingle-Pass Type I Membrane ProteinIsoform 2: Secreted |
| Alternative Protein Names | Inducible T-Cell Costimulator proteinActivation-Inducible Lymphocyte Immunomediatory Molecule proteinCd Antigen Cd278 proteinICOS proteinAILIM protein |
Information sourced from Uniprot.org