Human IBSP/Sialoprotein II protein (Recombinant) (His-Tag) (STJP019542)

SPECIFICATIONS
HostHEK293 Cells
ImmunogenHuman
STJP019542
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General Information

Short DescriptionRecombinant-Human IBSP/Sialoprotein II-His-Tag protein was developed from hek293 cells and has a target region of His-Tag. For use in research applications.
ApplicationsSDS-PAGE/Bioactivity
HostHEK293 Cells
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

Concentration0.5 mg/mL
FormulationLiquid in phosphate-Buffered Saline (pH 7.4) containing 10% Glycerol
Storage InstructionFor short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles.
Endotoxin< 1 EU per 1ug of protein (determined by LAL method)
ImmunoreactivityMeasured by the ability of the immobilized protein to support the adhesion of MCF7 human breast cancer cells. When cells are added to Human IBSP coated plates 3 ug/ml. This effect is more to 40%.

Target Information

Gene SymbolIBSP
Gene ID3381
Uniprot IDSIAL_HUMAN
Accession NumberNP_004958.2
ImmunogenHuman
Immunogen Region17-317aa
Immunogen SequenceFSMKNLHRRV KIEDSEENGV FKYRPRYYLY KHAYFYPHLK RFPVQGSSDS SEENGDDSSE EEEEEEETSN EGENNEESNE DEDSEAENTT LSATTLGYGE DATPGTGYTG LAAIQLPKKA GDITNKATKE KESDEEEEEE EEGNENEESE AEVDENEQGI NGTSTNSTEA ENGNGSSGGD NGEEGEEESV TGANAEDTTE TGRQGKGTSK TTTSPNGGFE PTTPPQVY

Additional Info

Tissue Specificity Expressed in bone (at protein level). Expressed in trophoblast cells of placenta (at protein level). Expressed in brain.
Post Translational Modifications N-glycosylated.glycans consist of sialylated and core-fucosylated bi-, tri- and tetraantennary chains. O-glycosylated at eight sites.mucin-type glycans contain Gal, GlcNAc, GalNAc and terminal NeuAc.
Function Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes adhesion and migration of various cells via the alpha-V/beta-3 integrin receptor (ITGAV:ITGB3).
Protein Name Integrin-Binding Sialoprotein
Bone Sialoprotein 2
Bone Sialoprotein Ii
Bsp Ii
Cell-Binding Sialoprotein
Database Links Reactome: R-HSA-216083
Reactome: R-HSA-3000178
Cellular Localisation Secreted
Alternative Protein Names Integrin-Binding Sialoprotein
Bone Sialoprotein 2 protein
Bone Sialoprotein Ii protein
Bsp Ii protein
Cell-Binding Sialoprotein
IBSP protein
BNSP protein

Information sourced from Uniprot.org

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