Name: Human Glyoxalase I protein (Recombinant) (His-Tag) (STJP019206)
SKU: STJP019206
Availability: InStock

Human Glyoxalase I protein (Recombinant) (His-Tag) (STJP019206)

SKU:

STJP019206

£87.50 - £361.50

Short Description :	Recombinant-Human Glyoxalase I-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications.
Applications:	Enzyme Activity/SDS-PAGE
Host:	E.coli
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Concentration:	1 mg/mL
Formulation:	Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% Glycerol
Storage Instruction:	For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles.
Endotoxin:	< 1 EU per 1ug of protein (determined by LAL method)
Immunoreactivity:	Specific activity: > 400unit/mg. One unit will form 1.0umol of S-lactoylgutathione from methylglyoxal and reduced glutathione per minute at pH6.5 at 25C.

Gene Symbol:	GLO1
Gene ID:	2739
Uniprot ID:	LGUL_HUMAN
Accession Number:	NP_006699
Immunogen:	Human
Immunogen Region:	1-184aa
Immunogen Sequence:	ARPCIPKSFG YSSVVCVCNA TYCDSFDPPT FPALGTFSRY ESTRSGRRME LSMGPIQANH TGTGLLLTLQ PEQKFQKVKG FGGAMTDAAA LNILALSPPA QNLLLKSYFS EEGIGYNIIR VPMASCDFSI RTYTYADTPD DFQLHNFSLP EEDTKLKIPL IHRALQLAQR PVSLLASPWT SPTWLKTNGA VNGKGSLKGQ PGDIYHQTWA RYFVKFLDAY AEHKLQFW

Post Translational Modifications	Glutathionylation at Cys-139 inhibits enzyme activity. Phosphorylated at Thr-107 in the presence of CaMK2. However, this is a consensus site for phosphorylation by CK2 so phosphorylation may be mediated by CK2 rather than CaMK2. Phosphorylation is induced by TNF and suppresses the TNF-induced transcriptional activity of NF-kappa-B. Exists in a nitric oxide (NO)-modified form. The exact nature of the modification is unknown, but it suppresses the TNF-induced transcriptional activity of NF-kappa-B.
Function	Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Involved in the regulation of TNF-induced transcriptional activity of NF-kappa-B. Required for normal osteoclastogenesis.
Protein Name	Lactoylglutathione Lyase Aldoketomutase Glyoxalase I Glx I Ketone-Aldehyde Mutase Methylglyoxalase S-D-Lactoylglutathione Methylglyoxal Lyase
Database Links	Reactome: R-HSA-70268
Cellular Localisation
Alternative Protein Names	Lactoylglutathione Lyase protein Aldoketomutase protein Glyoxalase I protein Glx I protein Ketone-Aldehyde Mutase protein Methylglyoxalase protein S-D-Lactoylglutathione Methylglyoxal Lyase protein GLO1 protein

Information sourced from Uniprot.org

Human Glyoxalase I protein (Recombinant) (His-Tag) (STJP019206)

General Information

Model	Name	Host	Application
STJP019636	Human Glyoxalase I protein (Recombinant) (No-Tag) (STJP019636)	E.coli	SDS-PAGE
STJP016947	Human Glyoxalase II protein (Recombinant) (His-Tag) (STJP016947)	E.coli	Enzyme Activity/SDS-PAGE
STJP016447	Human FABP2/I-FABP protein (Recombinant) (His-Tag) (STJP016447)	E.coli	SDS-PAGE
STJP017420	Human I-TAC/CXCL11 protein (Recombinant) (His-Tag) (STJP017420)	E.coli	SDS-PAGE
STJP017084	Human CCL1/I-309 protein (Recombinant) (His-Tag) (STJP017084)	E.coli	SDS-PAGE