Human Glyoxalase I protein (Recombinant) (His-Tag) (STJP019206)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human Glyoxalase I-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | Enzyme Activity/SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 1 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% Glycerol |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Endotoxin | < 1 EU per 1ug of protein (determined by LAL method) |
| Immunoreactivity | Specific activity: > 400unit/mg. One unit will form 1.0umol of S-lactoylgutathione from methylglyoxal and reduced glutathione per minute at pH6.5 at 25C. |
Target Information
| Gene Symbol | GLO1 |
| Gene ID | 2739 |
| Uniprot ID | LGUL_HUMAN |
| Accession Number | NP_006699 |
| Immunogen | Human |
| Immunogen Region | 1-184aa |
| Immunogen Sequence | ARPCIPKSFG YSSVVCVCNA TYCDSFDPPT FPALGTFSRY ESTRSGRRME LSMGPIQANH TGTGLLLTLQ PEQKFQKVKG FGGAMTDAAA LNILALSPPA QNLLLKSYFS EEGIGYNIIR VPMASCDFSI RTYTYADTPD DFQLHNFSLP EEDTKLKIPL IHRALQLAQR PVSLLASPWT SPTWLKTNGA VNGKGSLKGQ PGDIYHQTWA RYFVKFLDAY AEHKLQFW |
Additional Info
| Post Translational Modifications | Glutathionylation at Cys-139 inhibits enzyme activity. Phosphorylated at Thr-107 in the presence of CaMK2. However, this is a consensus site for phosphorylation by CK2 so phosphorylation may be mediated by CK2 rather than CaMK2. Phosphorylation is induced by TNF and suppresses the TNF-induced transcriptional activity of NF-kappa-B. Exists in a nitric oxide (NO)-modified form. The exact nature of the modification is unknown, but it suppresses the TNF-induced transcriptional activity of NF-kappa-B. |
| Function | Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Involved in the regulation of TNF-induced transcriptional activity of NF-kappa-B. Required for normal osteoclastogenesis. |
| Protein Name | Lactoylglutathione LyaseAldoketomutaseGlyoxalase IGlx IKetone-Aldehyde MutaseMethylglyoxalaseS-D-Lactoylglutathione Methylglyoxal Lyase |
| Database Links | Reactome: R-HSA-70268 |
| Cellular Localisation | |
| Alternative Protein Names | Lactoylglutathione Lyase proteinAldoketomutase proteinGlyoxalase I proteinGlx I proteinKetone-Aldehyde Mutase proteinMethylglyoxalase proteinS-D-Lactoylglutathione Methylglyoxal Lyase proteinGLO1 protein |
Information sourced from Uniprot.org