Human Glyoxalase I protein (Recombinant) (His-Tag) (STJP019206)

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STJP019206
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Host: E.coli
Applications: Enzyme Activity/SDS-PAGE
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description : Recombinant-Human Glyoxalase I-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications.
Formulation: Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% Glycerol
Concentration: 1 mg/mL
Storage Instruction: For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles.
Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
Immunoreactivity: Specific activity: > 400unit/mg. One unit will form 1.0umol of S-lactoylgutathione from methylglyoxal and reduced glutathione per minute at pH6.5 at 25C.
Gene Symbol: GLO1
Gene ID: 2739
Uniprot ID: LGUL_HUMAN
Immunogen Region: 1-184aa
Accession Number: NP_006699
Immunogen: Human
Immunogen Sequence: ARPCIPKSFG YSSVVCVCNA TYCDSFDPPT FPALGTFSRY ESTRSGRRME LSMGPIQANH TGTGLLLTLQ PEQKFQKVKG FGGAMTDAAA LNILALSPPA QNLLLKSYFS EEGIGYNIIR VPMASCDFSI RTYTYADTPD DFQLHNFSLP EEDTKLKIPL IHRALQLAQR PVSLLASPWT SPTWLKTNGA VNGKGSLKGQ PGDIYHQTWA RYFVKFLDAY AEHKLQFW
Post Translational Modifications Glutathionylation at Cys-139 inhibits enzyme activity. Phosphorylated at Thr-107 in the presence of CaMK2. However, this is a consensus site for phosphorylation by CK2 so phosphorylation may be mediated by CK2 rather than CaMK2. Phosphorylation is induced by TNF and suppresses the TNF-induced transcriptional activity of NF-kappa-B. Exists in a nitric oxide (NO)-modified form. The exact nature of the modification is unknown, but it suppresses the TNF-induced transcriptional activity of NF-kappa-B.
Function Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Involved in the regulation of TNF-induced transcriptional activity of NF-kappa-B. Required for normal osteoclastogenesis.
Protein Name Lactoylglutathione Lyase
Aldoketomutase
Glyoxalase I
Glx I
Ketone-Aldehyde Mutase
Methylglyoxalase
S-D-Lactoylglutathione Methylglyoxal Lyase
Database Links Reactome: R-HSA-70268
Cellular Localisation
Alternative Protein Names Lactoylglutathione Lyase protein
Aldoketomutase protein
Glyoxalase I protein
Glx I protein
Ketone-Aldehyde Mutase protein
Methylglyoxalase protein
S-D-Lactoylglutathione Methylglyoxal Lyase protein
GLO1 protein

Information sourced from Uniprot.org

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