Human GCNT1 protein (Recombinant-Active) (C-His) (STJP012064)
SPECIFICATIONS
HostMammalian Cells
ConjugationUnconjugated
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Active-Human GCNT1-C-His protein was developed from mammalian cells and has a target region of C-His. For use in research applications. |
| Applications | ELISA/WB |
| Host | Mammalian Cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Reconstitute in sterile water for a stock solution. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | <0.1 EU/Mu g of the protein by the LAL method. |
| Immunoreactivity | Measured by its ability to transfer GlcNAc from UDPGlcNAc to B1-3 galactosyl-Nacetyl galactosamine. The specific activity is >350 pmol/min/Mu g, as measured under the described conditions. |
Target Information
| Gene Symbol | GCNT1 |
| Gene ID | 2650 |
| Uniprot ID | GCNT1_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Arg33-His428 |
Additional Info
| Function | Glycosyltransferase that catalyzes the transfer of an N-acetylglucosamine (GlcNAc) moiety in beta1-6 linkage from UDP-GlcNAc onto mucin-type core 1 O-glycan to form the branched mucin-type core 2 O-glycan. The catalysis is metal ion-independent and occurs with inversion of the anomeric configuration of sugar donor. Selectively involved in synthesis of mucin-type core 2 O-glycans that serve as scaffolds for the display of selectin ligand sialyl Lewis X epitope by myeloid cells, with an impact on homeostasis and recruitment to inflammatory sites. Can also act on glycolipid substrates. Transfers GlcNAc moiety to GalGb4Cer globosides in a reaction step to the synthesis of stage-specific embryonic antigen 1 (SSEA-1) determinant. Can use Galbeta1-3GalNAcalpha1- and Galbeta1-3GalNAcbeta1- oligosaccharide derivatives as acceptor substrates. |
| Protein Name | Beta-1 -3-Galactosyl-O-Glycosyl-Glycoprotein Beta-1 -6-N-AcetylglucosaminyltransferaseCore 2 Beta-1 -6-N-AcetylglucosaminyltransferaseC2glcnactCore 2-Branching EnzymeCore2-Glcnac-TransferaseC2gntCore 2 GntLeukocyte Type Core 2 Beta-1 -6-N-AcetylglucosaminyltransferaseC2gnt-L |
| Database Links | Reactome: R-HSA-913709 |
| Cellular Localisation | Golgi Apparatus MembraneSingle-Pass Type Ii Membrane ProteinAlso Detected In The Trans-Golgi Network |
| Alternative Protein Names | Beta-1 -3-Galactosyl-O-Glycosyl-Glycoprotein Beta-1 -6-N-Acetylglucosaminyltransferase proteinCore 2 Beta-1 -6-N-Acetylglucosaminyltransferase proteinC2glcnact proteinCore 2-Branching Enzyme proteinCore2-Glcnac-Transferase proteinC2gnt proteinCore 2 Gnt proteinLeukocyte Type Core 2 Beta-1 -6-N-Acetylglucosaminyltransferase proteinC2gnt-L proteinGCNT1 proteinNACGT2 protein |
Information sourced from Uniprot.org