Human EPO (Erythropoietin) CLIA Kit for Quantitative Detection
General Information
| Short Description | This human EPO kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Applications | CLIA |
| Reactivity | Human |
| Sensitivity | 7.5pg/mL |
| Detection Limit | 12.5~800pg/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Chemiluminescence |
Target Information
| Gene Symbol | EPO |
| Gene ID | 2056 |
| Uniprot ID | EPO_HUMAN |
| Specificity | This kit recognizes Human EPO in samples. No significant cross-reactivity or interference between Human EPO and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Tissue Specificity | Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals. |
| Function | Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3. |
| Protein Name | ErythropoietinEpoetin |
| Database Links | Reactome: R-HSA-1234158Reactome: R-HSA-9006335Reactome: R-HSA-9027276Reactome: R-HSA-9027277Reactome: R-HSA-9027283Reactome: R-HSA-9027284 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Erythropoietin CLIA kitEpoetin CLIA kitEPO CLIA kit |
| Specificity | This kit recognizes Human EPO in samples. No significant cross-reactivity or interference between Human EPO and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 88-102 | 95 |
| EDTA plasma(n=8) | 94-107 | 102 |
| Cell culture media(n=8) | 95-111 | 103 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 40.84 | 104.28 | 326.11 | 39.08 | 94.62 | 296.04 |
| Standard deviation | 4.14 | 7.36 | 32.71 | 3.56 | 11.07 | 22.11 |
| CV (%) | 10.14 | 7.06 | 10.03 | 9.11 | 11.70 | 7.47 |
Citations
Product Review
Frequently Asked Questions
Common questions about Human EPO (Erythropoietin) CLIA Kit for Quantitative Detection
This kit is designed for the quantitative detection of Human EPO (Erythropoietin) in serum, plasma, and other biological fluids. Reactivity is confirmed for human samples only.
This kit has a detection range of 12.5 to 800 pg/mL and a sensitivity of 7.5 pg/mL.
This kit uses chemiluminescence (CLIA) as its detection method for quantitative measurement of Human EPO.
The total assay time is approximately 3.5 hours from start to finish.
No significant cross-reactivity or interference between Human EPO and its analogues was observed, based on the specificity data provided for this kit.
If unopened, this kit may be stored at 2 to 8 degrees Celsius for up to one month. If the kit will not be used within that period, components should be stored separately according to the component table provided in the manual.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.