Human DNAJB1 (DnaJ homolog subfamily B member 1) CLIA Kit for Quantitative Detection
SPECIFICATIONS
General Information
| Short Description | This human DNAJB1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Applications | CLIA |
| Reactivity | Human |
| Sensitivity | 18.75pg/mL |
| Detection Limit | 31.25~2000pg/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Chemiluminescence |
Target Information
| Gene Symbol | DNAJB1 |
| Gene ID | 3337 |
| Uniprot ID | DNJB1_HUMAN |
| Specificity | This kit recognizes Human HSP-40 in samples. No significant cross-reactivity or interference between Human HSP-40 and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Function | Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and recovery phase period of the heat shock response. Stimulates ATP hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B (in vitro). |
| Protein Name | Dnaj Homolog Subfamily B Member 1Dnaj Protein Homolog 1Heat Shock 40 Kda Protein 1Hsp40Heat Shock Protein 40Human Dnaj Protein 1Hdj-1 |
| Database Links | Reactome: R-HSA-3371453Reactome: R-HSA-3371497Reactome: R-HSA-3371568Reactome: R-HSA-3371571Reactome: R-HSA-5687128 |
| Cellular Localisation | CytoplasmNucleusNucleolusTranslocates Rapidly From The Cytoplasm To The NucleusAnd Especially To The NucleoliUpon Heat Shock |
| Alternative CLIA Names | Dnaj Homolog Subfamily B Member 1 CLIA kitDnaj Protein Homolog 1 CLIA kitHeat Shock 40 Kda Protein 1 CLIA kitHsp40 CLIA kitHeat Shock Protein 40 CLIA kitHuman Dnaj Protein 1 CLIA kitHdj-1 CLIA kitDNAJB1 CLIA kitDNAJ1 CLIA kitHDJ1 CLIA kitHSPF1 CLIA kit |
| Specificity | This kit recognizes Human HSP-40 in samples. No significant cross-reactivity or interference between Human HSP-40 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 93-106 | 100 |
| EDTA plasma(n=8) | 85-99 | 92 |
| Cell culture media(n=8) | 92-104 | 98 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 104.10 | 176.93 | 877.45 | 99.93 | 193.57 | 797.78 |
| Standard deviation | 9.47 | 14.90 | 83.80 | 12.28 | 23.17 | 48.51 |
| CV (%) | 9.10 | 8.42 | 9.55 | 12.29 | 11.97 | 6.08 |