Human DNAJB1 (DnaJ homolog subfamily B member 1) CLIA Kit for Quantitative Detection

Name: Human DNAJB1 (DnaJ homolog subfamily B member 1) CLIA Kit for Quantitative Detection
SKU: STJC0000810
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

STJC0000810

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Short Description	This human DNAJB1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.
Applications	CLIA
Reactivity	Human
Sensitivity	18.75pg/mL
Detection Limit	31.25~2000pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Chemiluminescence

Gene Symbol	DNAJB1
Gene ID	3337
Uniprot ID	DNJB1_HUMAN
Specificity	This kit recognizes Human HSP-40 in samples. No significant cross-reactivity or interference between Human HSP-40 and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Function	Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and recovery phase period of the heat shock response. Stimulates ATP hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B (in vitro).
Protein Name	Dnaj Homolog Subfamily B Member 1 Dnaj Protein Homolog 1 Heat Shock 40 Kda Protein 1 Hsp40 Heat Shock Protein 40 Human Dnaj Protein 1 Hdj-1
Database Links	Reactome: R-HSA-3371453 Reactome: R-HSA-3371497 Reactome: R-HSA-3371568 Reactome: R-HSA-3371571 Reactome: R-HSA-5687128
Cellular Localisation	Cytoplasm Nucleus Nucleolus Translocates Rapidly From The Cytoplasm To The Nucleus And Especially To The Nucleoli Upon Heat Shock
Alternative CLIA Names	Dnaj Homolog Subfamily B Member 1 CLIA kit Dnaj Protein Homolog 1 CLIA kit Heat Shock 40 Kda Protein 1 CLIA kit Hsp40 CLIA kit Heat Shock Protein 40 CLIA kit Human Dnaj Protein 1 CLIA kit Hdj-1 CLIA kit DNAJB1 CLIA kit DNAJ1 CLIA kit HDJ1 CLIA kit HSPF1 CLIA kit
Specificity	This kit recognizes Human HSP-40 in samples. No significant cross-reactivity or interference between Human HSP-40 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	104.10	176.93	877.45	99.93	193.57	797.78
Standard deviation	9.47	14.90	83.80	12.28	23.17	48.51
CV (%)	9.10	8.42	9.55	12.29	11.97	6.08