Human Dihydrolipoamide Dehydrogenase/DLD protein (Recombinant) (His-Tag) (STJP016646)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human Dihydrolipoamide Dehydrogenase/DLD-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE/Enzyme Activity |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 1 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 0.1M NaCl, 10% Glycerol |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Specific activity is > 200unit/mg, one unit will reduce 1.0 umole of DL-lipoamide to DL-dihydrolipoamide per minute at pH 6.5 at 25C. |
Target Information
| Gene Symbol | DLD |
| Gene ID | 1738 |
| Uniprot ID | DLDH_HUMAN |
| Accession Number | NP_000099 |
| Immunogen | Human |
| Immunogen Region | 36-509aa |
| Immunogen Sequence |
Additional Info
| Post Translational Modifications | Tyrosine phosphorylated. |
| Function | Lipoamide dehydrogenase is a component of the glycine cleavage system as well as an E3 component of three alpha-ketoacid dehydrogenase complexes (pyruvate-, alpha-ketoglutarate-, and branched-chain amino acid-dehydrogenase complex). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion. A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A. In monomeric form may have additional moonlighting function as serine protease. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction. |
| Protein Name | Dihydrolipoyl Dehydrogenase - MitochondrialDihydrolipoamide DehydrogenaseGlycine Cleavage System L Protein |
| Database Links | Reactome: R-HSA-204174Reactome: R-HSA-5362517Reactome: R-HSA-6783984Reactome: R-HSA-70895Reactome: R-HSA-9837999Reactome: R-HSA-9853506Reactome: R-HSA-9858328Reactome: R-HSA-9859138Reactome: R-HSA-9861559Reactome: R-HSA-9865113Reactome: R-HSA-9907570Reactome: R-HSA-9912481Reactome: R-HSA-9912529 |
| Cellular Localisation | Mitochondrion MatrixNucleusCell ProjectionCiliumFlagellumCytoplasmic VesicleSecretory VesicleAcrosomeMainly Localizes In The MitochondrionA Small Fraction Localizes To The NucleusWhere The 2-Oxoglutarate Dehydrogenase Complex Is Required For Histone Succinylation |
| Alternative Protein Names | Dihydrolipoyl Dehydrogenase - Mitochondrial proteinDihydrolipoamide Dehydrogenase proteinGlycine Cleavage System L Protein proteinDLD proteinGCSL proteinLAD proteinPHE3 protein |
Information sourced from Uniprot.org