Human Cystatin E/M protein (Recombinant) (His-Tag) (STJP019258)

SPECIFICATIONS
HostE.coli
ImmunogenHuman
STJP019258
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General Information

Short DescriptionRecombinant-Human Cystatin E/M-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications.
ApplicationsSDS-PAGE/Enzyme Activity
HostE.coli
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

Concentration0.5 mg/mL
FormulationLiquid in 20mM Tris-HCl buffer (pH 8.0) containing 10% Glycerol, 1mM DTT, 0.1M NaCl.
Storage InstructionFor short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles.
ImmunoreactivityThe IC50 value is < 20nM. The inhibitory function of Cystatin 6 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.

Target Information

Gene SymbolCST6
Gene ID1474
Uniprot IDCYTM_HUMAN
Accession NumberNP_001314.1
ImmunogenHuman
Immunogen Region29-149aa
Immunogen SequenceRPQERMVGE LRDLSPDDPQ VQKAAQAAVA SYNMGSNSIY YFRDTHIIKA QSQLVAGIKY FLTMEMGSTD CRKTRVTGDH VDLTTCPLAA GAQQEKLRCD FEVLVVPWQN SSQLLKHNCV QM

Additional Info

Tissue Specificity Restricted to the stratum granulosum of normal skin, the stratum granulosum/spinosum of psoriatic skin, and the secretory coils of eccrine sweat glands. Low expression levels are found in the nasal cavity.
Post Translational Modifications Substrate for transglutaminases. Acts as an acyl acceptor but not as an acyl donor.
Function High affinity inhibitor for cathepsin L, cathepsin L2 (cathepsin V), and legumain. Involved in the regulation of epidermal cornification, and hair follicle morphogenesis and maintenance.
Protein Name Cystatin-M
Cystatin-6
Cystatin-E
Database Links
Cellular Localisation Secreted
Alternative Protein Names Cystatin-M protein
Cystatin-6 protein
Cystatin-E protein
CST6 protein

Information sourced from Uniprot.org

Citations

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