Human CYP27B1 protein (Recombinant) (N-His) (STJP006543)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human CYP27B1-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | CYP27B1 |
| Gene ID | 1594 |
| Uniprot ID | CP27B_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Asp256-Arg508 |
Additional Info
| Function | A cytochrome P450 monooxygenase involved in vitamin D metabolism and in calcium and phosphorus homeostasis. Catalyzes the rate-limiting step in the activation of vitamin D in the kidney, namely the hydroxylation of 25-hydroxyvitamin D3/calcidiol at the C1alpha-position to form the hormonally active form of vitamin D3, 1alpha,25-dihydroxyvitamin D3/calcitriol that acts via the vitamin D receptor (VDR). Has 1alpha-hydroxylase activity on vitamin D intermediates of the CYP24A1-mediated inactivation pathway. Converts 24R,25-dihydroxyvitamin D3/secalciferol to 1-alpha,24,25-trihydroxyvitamin D3, an active ligand of VDR. Also active on 25-hydroxyvitamin D2. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via FDXR/adrenodoxin reductase and FDX1/adrenodoxin. |
| Protein Name | 25-Hydroxyvitamin D-1 Alpha Hydroxylase - Mitochondrial25-Ohd-1 Alpha-Hydroxylase25-Hydroxyvitamin D(3 1-Alpha-HydroxylaseVd3 1a HydroxylaseCalcidiol 1-MonooxygenaseCytochrome P450 Subfamily Xxviib Polypeptide 1Cytochrome P450c1 AlphaCytochrome P450vd1-AlphaCytochrome P450 27b1 |
| Database Links | Reactome: R-HSA-196791Reactome: R-HSA-211916Reactome: R-HSA-5579014 |
| Cellular Localisation | Mitochondrion Membrane |
| Alternative Protein Names | 25-Hydroxyvitamin D-1 Alpha Hydroxylase - Mitochondrial protein25-Ohd-1 Alpha-Hydroxylase protein25-Hydroxyvitamin D(3 1-Alpha-Hydroxylase proteinVd3 1a Hydroxylase proteinCalcidiol 1-Monooxygenase proteinCytochrome P450 Subfamily Xxviib Polypeptide 1 proteinCytochrome P450c1 Alpha proteinCytochrome P450vd1-Alpha proteinCytochrome P450 27b1 proteinCYP27B1 proteinCYP1ALPHA proteinCYP27B protein |
Information sourced from Uniprot.org