| Short Description : | Recombinant-Human Cyclophilin E/PPIE-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications: | SDS-PAGE/Enzyme Activity |
| Host: | E.coli |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Concentration: | 1 mg/mL |
| Formulation: | Liquid in 20mM Tris-HCl buffer (pH 8.0) |
| Storage Instruction: | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Endotoxin: | < 1 EU per 1ug of protein (determined by LAL method) |
| Immunoreactivity: | Specific activity is > 210nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-Hcl pH8.0 using chymotrypsin. |
| Gene Symbol: | PPIE |
| Gene ID: | 10450 |
| Uniprot ID: | PPIE_HUMAN |
| Accession Number: | NP_006103 |
| Immunogen: | Human |
| Immunogen Region: | 1-301aa |
| Immunogen Sequence: | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMATT KRVLYVGGLA EEVDDKVLHA AFIPFGDITD IQIPLDYETE KHRGFAFVEF ELAEDAAAAI DNMNESELFG RTIRVNLAKP MRIKEGSSRP VWSDDDWLKK FSGKTLEENK EEEGSEPPKA ETQEGEPIAK KARSNPQVYM DIKIGNKPAG RIQMLLRSDV VPMTAENFRC LCTHEKGFGF KGSSFHRI |
| Tissue Specificity | Found in all the examined tissues including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. |
| Function | Involved in pre-mRNA splicing as component of the spliceosome. Combines RNA-binding and PPIase activities. Binds mRNA and has a preference for single-stranded RNA molecules with poly-A and poly-U stretches, suggesting it binds to the poly(A)-region in the 3'-UTR of mRNA molecules. Catalyzes the cis-trans isomerization of proline imidic peptide bonds in proteins. Inhibits KMT2A activity.this requires proline isomerase activity. |
| Protein Name | Peptidyl-Prolyl Cis-Trans Isomerase EPpiase ECyclophilin ECyclophilin-33Rotamase E |
| Database Links | Reactome: R-HSA-6781823Reactome: R-HSA-6781827Reactome: R-HSA-6782135Reactome: R-HSA-6782210Reactome: R-HSA-6798695Reactome: R-HSA-72163 |
| Cellular Localisation | Nucleus |
| Alternative Protein Names | Peptidyl-Prolyl Cis-Trans Isomerase E proteinPpiase E proteinCyclophilin E proteinCyclophilin-33 proteinRotamase E proteinPPIE proteinCYP33 protein |
Information sourced from Uniprot.org
