Human Cyclophilin E/PPIE protein (Recombinant) (His-Tag) (STJP019730)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human Cyclophilin E/PPIE-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE/Enzyme Activity |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 1 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Endotoxin | < 1 EU per 1ug of protein (determined by LAL method) |
| Immunoreactivity | Specific activity is > 210nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-Hcl pH8.0 using chymotrypsin. |
Target Information
| Gene Symbol | PPIE |
| Gene ID | 10450 |
| Uniprot ID | PPIE_HUMAN |
| Accession Number | NP_006103 |
| Immunogen | Human |
| Immunogen Region | 1-301aa |
| Immunogen Sequence | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMATT KRVLYVGGLA EEVDDKVLHA AFIPFGDITD IQIPLDYETE KHRGFAFVEF ELAEDAAAAI DNMNESELFG RTIRVNLAKP MRIKEGSSRP VWSDDDWLKK FSGKTLEENK EEEGSEPPKA ETQEGEPIAK KARSNPQVYM DIKIGNKPAG RIQMLLRSDV VPMTAENFRC LCTHEKGFGF KGSSFHRI |
Additional Info
| Tissue Specificity | Found in all the examined tissues including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. |
| Function | Involved in pre-mRNA splicing as component of the spliceosome. Combines RNA-binding and PPIase activities. Binds mRNA and has a preference for single-stranded RNA molecules with poly-A and poly-U stretches, suggesting it binds to the poly(A)-region in the 3'-UTR of mRNA molecules. Catalyzes the cis-trans isomerization of proline imidic peptide bonds in proteins. Inhibits KMT2A activity.this requires proline isomerase activity. |
| Protein Name | Peptidyl-Prolyl Cis-Trans Isomerase EPpiase ECyclophilin ECyclophilin-33Rotamase E |
| Database Links | Reactome: R-HSA-6781823Reactome: R-HSA-6781827Reactome: R-HSA-6782135Reactome: R-HSA-6782210Reactome: R-HSA-6798695Reactome: R-HSA-72163 |
| Cellular Localisation | Nucleus |
| Alternative Protein Names | Peptidyl-Prolyl Cis-Trans Isomerase E proteinPpiase E proteinCyclophilin E proteinCyclophilin-33 proteinRotamase E proteinPPIE proteinCYP33 protein |
Information sourced from Uniprot.org