| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.09ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This CTSK Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
CTSK |
| Gene ID: |
1513 |
| Uniprot ID: |
CATK_HUMAN |
| Specificity: |
This kit recognizes Human CTSK in samples. No significant cross-reactivity or interference between Human CTSK and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Thiol protease involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation. Involved in the release of thyroid hormone thyroxine (T4) by limited proteolysis of TG/thyroglobulin in the thyroid follicle lumen. |
| Protein Name | Cathepsin KCathepsin OCathepsin O2Cathepsin X |
| Database Links | Reactome: R-HSA-1442490Reactome: R-HSA-1474228Reactome: R-HSA-1592389Reactome: R-HSA-1679131Reactome: R-HSA-2132295Reactome: R-HSA-8939242 |
| Cellular Localisation | LysosomeSecretedApical Cell MembranePeripheral Membrane ProteinExtracellular SideLocalizes To The Lumen Of Thyroid Follicles And To The Apical Membrane Of Thyroid Epithelial Cells |
| Alternative ELISA Names | Cathepsin K ELISA kitCathepsin O ELISA kitCathepsin O2 ELISA kitCathepsin X ELISA kitCTSK ELISA kitCTSO ELISA kitCTSO2 ELISA kit |
| Specificity | This kit recognizes Human CTSK in samples. No significant cross-reactivity or interference between Human CTSK and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 86-97 | 92 |
| EDTA plasma (n=8) | 88-104 | 95 |
| Cell culture media (n=8) | 94-107 | 100 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.46 | 1.57 | 4.41 | 0.48 | 1.52 | 4.13 |
| Standard deviation | 0.03 | 0.08 | 0.17 | 0.03 | 0.07 | 0.16 |
| CV (%) | 6.52 | 5.10 | 3.85 | 6.25 | 4.61 | 3.87 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
