Human CTSK (Cathepsin K) Sandwich ELISA Kit (STJE0002389)

Name: Human CTSK (Cathepsin K) Sandwich ELISA Kit (STJE0002389)
SKU: STJE0002389
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

STJE0002389

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Short Description	This CTSK Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Human
Sensitivity	0.09ng/mL
Detection Limit	0.16~10ng/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Gene Symbol	CTSK
Gene ID	1513
Uniprot ID	CATK_HUMAN
Specificity	This kit recognizes Human CTSK in samples. No significant cross-reactivity or interference between Human CTSK and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Function	Thiol protease involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation. Involved in the release of thyroid hormone thyroxine (T4) by limited proteolysis of TG/thyroglobulin in the thyroid follicle lumen.
Protein Name	Cathepsin K Cathepsin O Cathepsin O2 Cathepsin X
Database Links	Reactome: R-HSA-1442490 Reactome: R-HSA-1474228 Reactome: R-HSA-1592389 Reactome: R-HSA-1679131 Reactome: R-HSA-2132295 Reactome: R-HSA-8939242
Cellular Localisation	Lysosome Secreted Apical Cell Membrane Peripheral Membrane Protein Extracellular Side Localizes To The Lumen Of Thyroid Follicles And To The Apical Membrane Of Thyroid Epithelial Cells
Alternative ELISA Names	Cathepsin K ELISA kit Cathepsin O ELISA kit Cathepsin O2 ELISA kit Cathepsin X ELISA kit CTSK ELISA kit CTSO ELISA kit CTSO2 ELISA kit
Specificity	This kit recognizes Human CTSK in samples. No significant cross-reactivity or interference between Human CTSK and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	0.46	1.57	4.41	0.48	1.52	4.13
Standard deviation	0.03	0.08	0.17	0.03	0.07	0.16
CV (%)	6.52	5.10	3.85	6.25	4.61	3.87