Human CTACK (Cutaneous T-cell Attracting Chemokine) Sandwich ELISA Kit (STJE0019888)

SPECIFICATIONS
STJE0019888
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General Information

Short DescriptionThis CTACK Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
ApplicationsELISA
ReactivityHuman
Sensitivity0.62 pg/mL
Detection Limit1.56-100 pg/mL
NoteFOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

Storage InstructionIf unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time3.5h
DetectionColormetric

Target Information

Gene SymbolCCL27
Gene ID10850
Uniprot IDCCL27_HUMAN
SpecificityThis kit recognizes Human CTACK in samples. No significant cross-reactivity or interference between Human CTACK and analogues was observed.
Sample Typeserum, plasma and other biological fluids

Additional Info

Tissue Specificity Testis, thymus, placenta, ovary and skin.
Function Chemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. Binds to CCR10.
Protein Name C-C Motif Chemokine 27
Cc Chemokine Ilc
Cutaneous T-Cell-Attracting Chemokine
Ctack
Eskine
Il-11 R-Alpha-Locus Chemokine
Skinkine
Small-Inducible Cytokine A27
Database Links Reactome: R-HSA-380108
Reactome: R-HSA-418594
Cellular Localisation Secreted
Alternative ELISA Names C-C Motif Chemokine 27 ELISA kit
Cc Chemokine Ilc ELISA kit
Cutaneous T-Cell-Attracting Chemokine ELISA kit
Ctack ELISA kit
Eskine ELISA kit
Il-11 R-Alpha-Locus Chemokine ELISA kit
Skinkine ELISA kit
Small-Inducible Cytokine A27 ELISA kit
CCL27 ELISA kit
ILC ELISA kit
SCYA27 ELISA kit
Specificity This kit recognizes Human CTACK in samples. No significant cross-reactivity or interference between Human CTACK and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-103 94
EDTA plasma (n=8) 92-103 98
Cell culture media (n=8) 94-107 101

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 5.36 8.56 38.57 5.54 8.19 38.27
Standard deviation 0.29 0.43 1.89 0.29 0.40 2.03
CV (%) 5.37 5.03 4.91 5.22 4.94 5.31

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