Human Complement C1r subcomponent protein (Recombinant) (C-His) (STJP000917)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human Complement C1r subcomponent Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Asp705) of human Complement C1r subcomponent (Accession #NP_001724.3) fused with a 6×His tag at t
General Information
| Short Description | Recombinant-Human Complement C1r subcomponent-C-His protein was developed in hek293 cells using the region Met1-Asp705. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Gly-Arg-ThioBenzyl ester (Z-GR-SBzl) , in the presence of 5, 5 NA Dithio-bis (2-nitrobenzoic acid) (DTNB).The specific activity is >2828 pmol/min/Mu g, as measured u |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | C1R |
| Gene ID | 715 |
| Uniprot ID | C1R_HUMAN |
| Immunogen | Recombinant Human Complement C1r subcomponent Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Asp705) of human Complement C1r subcomponent (Accession #NP_001724.3) fused with a 6×His tag at t |
| Immunogen Region | Met1-Asp705 |
| Immunogen Sequence | MWLLYLLVPALFCRAGGSIP IPQKLFGEVTSPLFPKPYPN NFETTTVITVPTGYRVKLVF QQFDLEPSEGCFYDYVKISA DKKSLGRFCGQLGSPLGNPP GKKEFMSQGNKMLLTFHTDF SNEENGTIMFYKGFLAYYQA VDLDECASRSKLGEEDPQPQ CQHLCHNYVGGYFCSCRPGY ELQEDRHSCQAECSSELYTE ASGYISSLEYPRSYPPDLRC NYSIRVERGLTLHLKFLEP |
Additional Info
| Post Translational Modifications | Cleaved and activated by autocatalytic processing to generate Complement C1r subcomponent heavy and light chains that are connected by disulfide bonds. The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. |
| Function | Serine protease component of the complement C1 complex, a multiprotein complex that initiates the classical pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. C1R catalyzes the first enzymatic step in the classical complement pathway: it is activated by the C1Q subcomplex of the C1 complex, which associates with IgG or IgM immunoglobulins complexed with antigens to form antigen-antibody complexes on the surface of pathogens. Immunoglobulin-binding promotes the autocatalytic cleavage and activation of C1R. Activated C1R then cleaves and activates C1S, the second protease of the classical complement pathway. It is unclear if C1R activates C1S within single, strained C1 complexes or between neighboring C1 complexes on surfaces. |
| Protein Name | Complement C1r SubcomponentComplement Component 1 Subcomponent R Cleaved Into - Complement C1r Subcomponent Heavy ChainComplement C1r Subcomponent Chain A - Complement C1r Subcomponent Light ChainComplement C1r Subcomponent Chain B |
| Database Links | Reactome: R-HSA-166663Reactome: R-HSA-173623Reactome: R-HSA-977606 |
| Cellular Localisation | SecretedCell SurfaceRecruited To The Surface Of Pathogens By The C1q Subcomplex |
| Alternative Protein Names | Complement C1r Subcomponent proteinComplement Component 1 Subcomponent R Cleaved Into - Complement C1r Subcomponent Heavy Chain proteinComplement C1r Subcomponent Chain A - Complement C1r Subcomponent Light Chain proteinComplement C1r Subcomponent Chain B proteinC1R protein |
Information sourced from Uniprot.org