Human CD301 protein (Recombinant) (C-His) (STJP000979)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human CD301 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln61-His316) of human CLEC10A/MGL1/CD301 (Accession #NP_878910.1) fused with a 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human CD301-C-His protein was developed in hek293 cells using the region Gln61-His316. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | < 1.0 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | CLEC10A |
| Gene ID | 10462 |
| Uniprot ID | CLC10_HUMAN |
| Immunogen | Recombinant Human CD301 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln61-His316) of human CLEC10A/MGL1/CD301 (Accession #NP_878910.1) fused with a 6×His tag at the C-terminus. |
| Immunogen Region | Gln61-His316 |
| Immunogen Sequence | QNSKFQRDLVTLRTDFSNFT SNTVAEIQALTSQGSSLEET IASLKAEVEGFKQERQAGVS ELQEHTTQKAHLGHCPHCPS VCVPVHSEMLLRVQQLVQDL KKLTCQVATLNNNASTEGTC CPVNWVEHQDSCYWFSHSGM SWAEAEKYCQLKNAHLVVIN SREEQNFVQKYLGSAYTWMG LSDPEGAWKWVDGTDYATGF QNWKPGQPDDWQGHGLGGGE DCAHFHPDGRWNDDVCQRP |
Additional Info
| Tissue Specificity | Expressed in myeloid antigen presenting cells in lymph nodes and skin (at protein level). Expressed in dermal dendritic cells (at protein level). |
| Function | C-type lectin receptor involved in recognition of N-acetylgalactosamine (GalNAc)-terminated glycans by myeloid antigen presenting cells (APCs). Binds in a Ca(2+)-dependent manner to alpha- and beta-linked GalNAc residues on glycoprotein and glycolipid antigens, including alphaGalNAc- and Galbeta1->3GalNAc-O-Ser/Thr also known as Tn and T antigens, LacdiNAc epitope GalNAcbeta1->4GlcNAc and its derivative GalNAcbeta1->4-(Fucalpha1->3)GlcNAc, O-linked core 5 and 6 glycans, and GM2 and GD2 gangliosides. Acts as a signaling receptor at the interface of APC-T cell interactions. On immature dendritic cells, recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and downregulates PTRPN/CD45 phosphatase activity with an impact on T cell activation threshold, cytokine production and proliferation. Modulates dendritic cell maturation toward a tolerogenic phenotype leading to generation of regulatory CD4-positive T cell subset with immune suppressive functions. Acts as an endocytic pattern recognition receptor involved in antitumor immunity. During tumorigenesis, recognizes Tn antigens and its sialylated forms Neu5Ac-Tn and Neu5Gc-Tn expressed on tumor cell mucins. On immature dendritic cells, can internalize Tn-terminated immunogens and target them to endolysosomal compartment for MHC class I and II antigen presentation to CD8-positive and CD4-positive T cells, respectively. |
| Protein Name | C-Type Lectin Domain Family 10 Member AC-Type Lectin Superfamily Member 14Dc-Asialoglycoprotein ReceptorDc-AsgprMacrophage Galactose-Type LectinMglMacrophage Lectin 2Cd Antigen Cd301 |
| Database Links | Reactome: R-HSA-5621480 |
| Cellular Localisation | Cell MembraneSingle-Pass Type Ii Membrane ProteinEarly Endosome MembraneLysosome MembraneRecycles Between The Plasma Membrane And The Endolysosomal CompartmentUpon Antigen BindingInternalizes Via Endocytosis And Then Dissociates From Antigen At Acidic Ph Characteristic Of Endolysosomal Vesicles |
| Alternative Protein Names | C-Type Lectin Domain Family 10 Member A proteinC-Type Lectin Superfamily Member 14 proteinDc-Asialoglycoprotein Receptor proteinDc-Asgpr proteinMacrophage Galactose-Type Lectin proteinMgl proteinMacrophage Lectin 2 proteinCd Antigen Cd301 proteinCLEC10A proteinCLECSF13 proteinCLECSF14 proteinHML protein |
Information sourced from Uniprot.org