| Host: | HEK293 |
| Note: | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-Human Carboxypeptidase A1-protein was developed from hek293. For use in research applications. |
| Conjugation: | Unconjugated |
| Formulation: | Lyophilised from 0.2 Mu m filtered PBS solution, pH7.2, 5% Trehalose. |
| Dilution Range: | Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Storage Instruction: | Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin: | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Immunoreactivity: | The activity was tested by the ability to cleave a fluorogenic substrate Ac-Phe-Thiaphe-OH in the presence of 5, 5 Dithio-bis. The specific activity was measured to be 4 nmole/ug/min NA |
| Gene Symbol: | CPA1 |
| Gene ID: | 1357 |
| Uniprot ID: | CBPA1_HUMAN |
| Immunogen Region: | LYS17-TYR417 |
| Immunogen: | Optimized DNA sequence encoding Human Carboxypeptidase A1 (LYS17-TYR417) preprotein including a C-terminal His tag was expressed in HEK293 cells. NA |
| Function | Carboxypeptidase that catalyzes the release of a C-terminal amino acid, but has little or no action with -Asp, -Glu, -Arg, -Lys or -Pro. Catalyzes the conversion of leukotriene C4 to leukotriene F4 via the hydrolysis of an amide bond. |
| Protein Name | Carboxypeptidase A1 |
| Database Links | Reactome: R-HSA-9925561 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Carboxypeptidase A1 proteinCPA1 proteinCPA protein |
Information sourced from Uniprot.org
