Human Carboxypeptidase A1 protein (Recombinant) (STJP000433)
SPECIFICATIONS
HostHEK293
ConjugationUnconjugated
ImmunogenOptimized DNA sequence encoding Human Carboxypeptidase A1 (LYS17-TYR417) preprotein including a C-terminal His tag was expressed in HEK293 cells. NA
General Information
| Short Description | Recombinant-Human Carboxypeptidase A1-protein was developed from hek293. For use in research applications. |
| Host | HEK293 |
| Note | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Formulation | Lyophilised from 0.2 Mu m filtered PBS solution, pH7.2, 5% Trehalose. |
| Storage Instruction | Can be stored in working aliquots at 2ยฐC-8ยฐC C for one month, or at-20ยฐC to-70ยฐC for 1 year. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ยตg (1EU/ยตg). NA |
| Immunoreactivity | The activity was tested by the ability to cleave a fluorogenic substrate Ac-Phe-Thiaphe-OH in the presence of 5, 5 Dithio-bis. The specific activity was measured to be 4 nmole/ug/min NA |
Target Information
| Gene Symbol | CPA1 |
| Gene ID | 1357 |
| Uniprot ID | CBPA1_HUMAN |
| Immunogen | Optimized DNA sequence encoding Human Carboxypeptidase A1 (LYS17-TYR417) preprotein including a C-terminal His tag was expressed in HEK293 cells. NA |
| Immunogen Region | LYS17-TYR417 |
Additional Info
| Function | Carboxypeptidase that catalyzes the release of a C-terminal amino acid, but has little or no action with -Asp, -Glu, -Arg, -Lys or -Pro. Catalyzes the conversion of leukotriene C4 to leukotriene F4 via the hydrolysis of an amide bond. |
| Protein Name | Carboxypeptidase A1 |
| Database Links | Reactome: R-HSA-9925561 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Carboxypeptidase A1 proteinCPA1 proteinCPA protein |
Information sourced from Uniprot.org