Human Carbonic anhydrase 12 protein (Active Recombinant) (C-His) (STJP000932)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human Carbonic anhydrase 12 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ala25-Gln291) of human Carbonic Anhydrase XII/CA12 (Accession #NP_001209.1) fused with a 6×His tag at the C-termin
General Information
| Short Description | Active Recombinant-Human Carbonic anhydrase 12-C-His protein was developed in hek293 cells using the region Ala25-Gln291. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its esterase activity. The specific activity is >48 pmoles/min/Mu g, as measured with 1 mM 4-Nitrophenyl acetate and 1 Mu g enzyme at 400 nm in 100 Mu L of 12.5 mM Tris, 75 mM NaCl, pH 7.5. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | CA12 |
| Gene ID | 771 |
| Uniprot ID | CAH12_HUMAN |
| Immunogen | Recombinant Human Carbonic anhydrase 12 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ala25-Gln291) of human Carbonic Anhydrase XII/CA12 (Accession #NP_001209.1) fused with a 6×His tag at the C-termin |
| Immunogen Region | Ala25-Gln291 |
| Immunogen Sequence | APVNGSKWTYFGPDGENSWS KKYPSCGGLLQSPIDLHSDI LQYDASLTPLEFQGYNLSAN KQFLLTNNGHSVKLNLPSDM HIQGLQSRYSATQLHLHWGN PNDPHGSEHTVSGQHFAAEL HIVHYNSDLYPDASTASNKS EGLAVLAVLIEMGSFNPSYD KIFSHLQHVKYKGQEAFVPG FNIEELLPERTAEYYRYRGS LTTPPCNPTVLWTVFRNPVQ ISQEQLLALETALYCTHMD |
Additional Info
| Tissue Specificity | Highly expressed in colon, kidney, prostate, intestine and activated lymphocytes. Expressed at much higher levels in the renal cell cancers than in surrounding normal kidney tissue. Moderately expressed in pancreas, ovary and testis. Expressed in sweat glands and bronchiolar epithelium. |
| Function | Reversible hydration of carbon dioxide. |
| Protein Name | Carbonic Anhydrase 12Carbonate Dehydratase XiiCarbonic Anhydrase XiiCa-XiiTumor Antigen Hom-Rcc-3.1.3 |
| Database Links | Reactome: R-HSA-1475029 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane ProteinCell Membrane |
| Alternative Protein Names | Carbonic Anhydrase 12 proteinCarbonate Dehydratase Xii proteinCarbonic Anhydrase Xii proteinCa-Xii proteinTumor Antigen Hom-Rcc-3.1.3 proteinCA12 protein |
Information sourced from Uniprot.org