| Host: |
HEK293 |
| Note: |
STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Human BACE1-protein was developed from hek293. For use in research applications. |
| Conjugation: |
Unconjugated |
| Formulation: |
Lyophilised from 0.2 Mu m filtered PBS solution, pH7.2, 5% Trehalose. |
| Storage Instruction: |
Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin: |
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Immunoreactivity: |
The activity was tested by the ability to digest a fluorogenic substrate and measuered to be 4 pmoles/min/ug NA |
| Gene Symbol: |
BACE1 |
| Gene ID: |
23621 |
| Uniprot ID: |
BACE1_HUMAN |
| Immunogen Region: |
THR22-THR457 |
| Immunogen: |
Optimized DNA sequence encoding extracellular domain of Human beta Site Amyloid Precursor Protein-BACE1 (THR22-THR457 ) including a C-terminal His tag was expressed in HEK293 cells. NA |
| Tissue Specificity | Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata. |
| Post Translational Modifications | N-Glycosylated. Addition of a bisecting N-acetylglucosamine by MGAT3 blocks lysosomal targeting, further degradation and is required for maintaining stability under stress conditions. Acetylated in the endoplasmic reticulum at Lys-126, Lys-275, Lys-279, Lys-285, Lys-299, Lys-300 and Lys-307. Acetylation by NAT8 and NAT8B is transient and deacetylation probably occurs in the Golgi. Acetylation regulates the maturation, the transport to the plasma membrane, the stability and the expression of the protein. Palmitoylation mediates lipid raft localization. Ubiquitinated at Lys-501, ubiquitination leads to lysosomal degradation. Monoubiquitinated and 'Lys-63'-linked polyubitinated. Deubiquitnated by USP8.inhibits lysosomal degradation. Phosphorylation at Ser-498 is required for interaction with GGA1 and retrograded transport from endosomal compartments to the trans-Golgi network. Non-phosphorylated BACE1 enters a direct recycling route to the cell surface. |
| Function | Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. Cleaves CHL1. |
| Protein Name | Beta-Secretase 1Aspartyl Protease 2Asp2Asp 2Beta-Site Amyloid Precursor Protein Cleaving Enzyme 1Beta-Site App Cleaving Enzyme 1Memapsin-2Membrane-Associated Aspartic Protease 2 |
| Database Links | Reactome: R-HSA-977225 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinGolgi ApparatusTrans-Golgi NetworkEndoplasmic ReticulumEndosomeCell SurfaceCytoplasmic Vesicle MembraneMembrane RaftLysosomeLate EndosomeEarly EndosomeRecycling EndosomeCell ProjectionAxonDendritePredominantly Localized To The Later Golgi/Trans-Golgi Network (Tgn) And Minimally Detectable In The Early Golgi CompartmentsA Small Portion Is Also Found In The Endoplasmic ReticulumEndosomes And On The Cell SurfaceColocalization With App In Early Endosomes Is Due To Addition Of Bisecting N-Acetylglucosamine Wich Blocks Targeting To Late Endosomes And LysosomesRetrogradly Transported From Endosomal Compartments To The Trans-Golgi Network In A Phosphorylation- And Gga1- Dependent Manner |
| Alternative Protein Names | Beta-Secretase 1 proteinAspartyl Protease 2 proteinAsp2 proteinAsp 2 proteinBeta-Site Amyloid Precursor Protein Cleaving Enzyme 1 proteinBeta-Site App Cleaving Enzyme 1 proteinMemapsin-2 proteinMembrane-Associated Aspartic Protease 2 proteinBACE1 proteinBACE proteinKIAA1149 protein |
Information sourced from Uniprot.org
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