Human BACE1 protein (Recombinant) (STJP000564)

Conjugation:	Unconjugated
Formulation:	Lyophilised from 0.2 Mu m filtered PBS solution, pH7.2, 5% Trehalose.
Storage Instruction:	Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA
Endotoxin:	Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA
Immunoreactivity:	The activity was tested by the ability to digest a fluorogenic substrate and measuered to be 4 pmoles/min/ug NA

Gene Symbol:	BACE1
Gene ID:	23621
Uniprot ID:	BACE1_HUMAN
Immunogen Region:	THR22-THR457
Immunogen:	Optimized DNA sequence encoding extracellular domain of Human beta Site Amyloid Precursor Protein-BACE1 (THR22-THR457 ) including a C-terminal His tag was expressed in HEK293 cells. NA

Tissue Specificity	Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata.
Post Translational Modifications	N-Glycosylated. Addition of a bisecting N-acetylglucosamine by MGAT3 blocks lysosomal targeting, further degradation and is required for maintaining stability under stress conditions. Acetylated in the endoplasmic reticulum at Lys-126, Lys-275, Lys-279, Lys-285, Lys-299, Lys-300 and Lys-307. Acetylation by NAT8 and NAT8B is transient and deacetylation probably occurs in the Golgi. Acetylation regulates the maturation, the transport to the plasma membrane, the stability and the expression of the protein. Palmitoylation mediates lipid raft localization. Ubiquitinated at Lys-501, ubiquitination leads to lysosomal degradation. Monoubiquitinated and 'Lys-63'-linked polyubitinated. Deubiquitnated by USP8.inhibits lysosomal degradation. Phosphorylation at Ser-498 is required for interaction with GGA1 and retrograded transport from endosomal compartments to the trans-Golgi network. Non-phosphorylated BACE1 enters a direct recycling route to the cell surface.
Function	Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. Cleaves CHL1.
Protein Name	Beta-Secretase 1 Aspartyl Protease 2 Asp2 Asp 2 Beta-Site Amyloid Precursor Protein Cleaving Enzyme 1 Beta-Site App Cleaving Enzyme 1 Memapsin-2 Membrane-Associated Aspartic Protease 2
Database Links	Reactome: R-HSA-977225
Cellular Localisation	Cell Membrane Single-Pass Type I Membrane Protein Golgi Apparatus Trans-Golgi Network Endoplasmic Reticulum Endosome Cell Surface Cytoplasmic Vesicle Membrane Membrane Raft Lysosome Late Endosome Early Endosome Recycling Endosome Cell Projection Axon Dendrite Predominantly Localized To The Later Golgi/Trans-Golgi Network (Tgn) And Minimally Detectable In The Early Golgi Compartments A Small Portion Is Also Found In The Endoplasmic Reticulum Endosomes And On The Cell Surface Colocalization With App In Early Endosomes Is Due To Addition Of Bisecting N-Acetylglucosamine Wich Blocks Targeting To Late Endosomes And Lysosomes Retrogradly Transported From Endosomal Compartments To The Trans-Golgi Network In A Phosphorylation- And Gga1- Dependent Manner
Alternative Protein Names	Beta-Secretase 1 protein Aspartyl Protease 2 protein Asp2 protein Asp 2 protein Beta-Site Amyloid Precursor Protein Cleaving Enzyme 1 protein Beta-Site App Cleaving Enzyme 1 protein Memapsin-2 protein Membrane-Associated Aspartic Protease 2 protein BACE1 protein BACE protein KIAA1149 protein

Information sourced from Uniprot.org

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