Human Aprataxin/APTX protein (Recombinant) (His-Tag) (STJP016620)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human Aprataxin/APTX-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 0.25 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 7.5) containing 20% Glycerol, 100mM NaCl, 0.1mM PMSF |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | APTX |
| Gene ID | 54840 |
| Uniprot ID | APTX_HUMAN |
| Accession Number | NP_778241 |
| Immunogen | Human |
| Immunogen Region | 1-168aa |
| Immunogen Sequence | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWAGSMQD PKMQVYKDEQ VVVIKDKYPK ARYHWLVLPW TSISSLKAVA REHLELLKHM HTVGEKVIVD FAGSSKLRFR LGYHAIPSMS HVHLHVISQD FDSPCLKNKK HWNSFNTEYF LESQAVIEMV QEAGRVTVRD GMPELLKLPL RCHECQQLLP SIPQLKEHLR KHWTQ |
Additional Info
| Tissue Specificity | Widely expressed.detected in liver, kidney and lymph node (at protein level). Isoform 1 is highly expressed in the cerebral cortex and cerebellum, compared to isoform 2 (at protein level). Widely expressed.detected throughout the brain, in liver, kidney, skeletal muscle, fibroblasts, lymphocytes and pancreas. |
| Function | DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair. Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non-ligatable breaks induced by reactive oxygen species. Catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'-phosphate termini that can be efficiently rejoined. Also able to hydrolyze adenosine 5'-monophosphoramidate (AMP-NH(2)) and diadenosine tetraphosphate (AppppA), but with lower catalytic activity. Likewise, catalyzes the release of 3'-linked guanosine (DNAppG) and inosine (DNAppI) from DNA, but has higher specific activity with 5'-linked adenosine (AppDNA). |
| Protein Name | AprataxinForkhead-Associated Domain Histidine Triad-Like ProteinFha-Hit |
| Database Links | |
| Cellular Localisation | NucleusNucleoplasmNucleolusUpon Genotoxic StressColocalizes With Xrcc1 At Sites Of Dna DamageColocalizes With Mdc1 At Sites Of Dna Double-Strand BreaksInteraction With Ncl Is Required For Nucleolar LocalizationIsoform 12: Cytoplasm |
| Alternative Protein Names | Aprataxin proteinForkhead-Associated Domain Histidine Triad-Like Protein proteinFha-Hit proteinAPTX proteinAXA1 protein |
Information sourced from Uniprot.org