Human Aldo-keto reductase 7A2/AKR7A2 protein (Recombinant) (His-Tag) (STJP016619)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human Aldo-keto reductase 7A2/AKR7A2-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE/Enzyme Activity |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 0.5 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% Glycerol |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Specific activity is > 1000pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0pmole of 1, 2-Naphthoquinone presence of NADPH per minute at pH 7.0 at 25C. |
Target Information
| Gene Symbol | AKR7A2 |
| Gene ID | 8574 |
| Uniprot ID | ARK72_HUMAN |
| Accession Number | NP_003680 |
| Immunogen | Human |
| Immunogen Region | 1-359aa |
| Immunogen Sequence | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSELEM LSAASRVVSR AAVHCALRSP PPEARALAMS RPPPPRVASV LGTMEMGRRM DAPASAAAVR AFLERGHTEL DTAFMYSDGQ SETILGGLGL GLGGGDCRVK IATKANPWDG KSLKPDSVRS QLETSLKRLQ CPQVDLFYLH APDHGTPVEE TLHACQRLHQ EGKFVELGLS NYASWEVAEI CTLCKSNG |
Additional Info
| Tissue Specificity | Detected in brain, liver, small intestine and testis, and at lower levels in heart, prostate, skeletal muscle and spleen. Detected in kidney proximal and distal tubules, endothelial cells lining the Bowman's capsules and some cysts. Detected at low levels in lung and pancreas (at protein level). Widely expressed. |
| Function | Catalyzes the NADPH-dependent reduction of succinic semialdehyde to gamma-hydroxybutyrate. May have an important role in producing the neuromodulator gamma-hydroxybutyrate (GHB). Has broad substrate specificity. Has NADPH-dependent aldehyde reductase activity towards 2-carboxybenzaldehyde, 2-nitrobenzaldehyde and pyridine-2-aldehyde (in vitro). Can reduce 1,2-naphthoquinone and 9,10-phenanthrenequinone (in vitro). Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. May be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen. |
| Protein Name | Aflatoxin B1 Aldehyde Reductase Member 2Afb1 Aldehyde Reductase 1Afb1-Ar 1Aldoketoreductase 7Succinic Semialdehyde ReductaseSsa Reductase |
| Database Links | Reactome: R-HSA-5423646 |
| Cellular Localisation | MitochondrionGolgi ApparatusCytoplasm |
| Alternative Protein Names | Aflatoxin B1 Aldehyde Reductase Member 2 proteinAfb1 Aldehyde Reductase 1 proteinAfb1-Ar 1 proteinAldoketoreductase 7 proteinSuccinic Semialdehyde Reductase proteinSsa Reductase proteinAKR7A2 proteinAFAR proteinAFAR1 proteinAKR7 protein |
Information sourced from Uniprot.org