DDX6 Positive Control for STJ500746 is synthetically produced from the sequence and is suitable for use in western blot applications.
Applications
WB
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Dilution Range
WB: 1:500
Formulation
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is
Storage Instruction
Store at-20°C for long term storage. Avoid freeze-thaw cycles.
This is positive control is recommended for use in combination with DDX6 antibody STJ500746.
Additional Info
Tissue Specificity
Abundantly expressed in most tissues.
Post Translational Modifications
Sumoylated.
Function
Essential for the formation of P-bodies, cytosolic membrane-less ribonucleoprotein granules involved in RNA metabolism through the coordinated storage of mRNAs encoding regulatory functions. Plays a role in P-bodies to coordinate the storage of translationally inactive mRNAs in the cytoplasm and prevent their degradation. In the process of mRNA degradation, plays a role in mRNA decapping. Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts.
CytoplasmP-BodyNucleusCytoplasmic Ribonucleoprotein GranuleImported In The Nucleus Via Interaction With Eif4enif1/4e-T Via A Piggy-Back MechanismUpon Cellular StressRelocalizes To Stress Granules
Alternative Peptide Names
Probable Atp-Dependent Rna Helicase Ddx6 proteinAtp-Dependent Rna Helicase P54 proteinDead Box Protein 6 proteinOncogene Rck proteinDDX6 proteinHLR2 proteinRCK protein
