| Host: |
Rabbit |
| Applications: |
WB/IF/ICC/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ZWINT |
| Gene ID: |
11130 |
| Uniprot ID: |
ZWINT_HUMAN |
| Immunogen Region: |
1-277 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-277 of human ZWINT (NP_008988.2). |
| Immunogen Sequence: |
MEAAETEAEAAALEVLAEVA GILEPVGLQEEAELPAKILV EFVVDSQKKDKLLCSQLQVA DFLQNILAQEDTAKGLDPLA SEDTSRQKAIAAKEQWKELK ATYREHVEAIKIGLTKALTQ MEEAQRKRTQLREAFEQLQA KKQMAMEKRRAVQNQWQLQQ EKHLQHLAEVSAEVRERKTG TQQELDRVFQKLGNLKQQAE QERDKLQRYQTFLQLLYTLQ GKLLFPEAEAEAENLPDDK |
| Function | Acts as a component of the outer kinetochore KNL1 complex that serves as a docking point for spindle assembly checkpoint components and mediates microtubule-kinetochore interactions. Kinetochores, consisting of a centromere-associated inner segment and a microtubule-contacting outer segment, play a crucial role in chromosome segregation by mediating the physical connection between centromeric DNA and spindle microtubules. The outer kinetochore is made up of the ten-subunit KMN network, comprising the MIS12, NDC80 and KNL1 complexes, and auxiliary microtubule-associated components.together they connect the outer kinetochore with the inner kinetochore, bind microtubules, and mediate interactions with mitotic checkpoint proteins that delay anaphase until chromosomes are bioriented on the spindle. Targets the RZZ complex to the kinetochore at prometaphase. Recruits MAD2L1 to the kinetochore, but is not required for BUB1B localization. In addition to orienting mitotic chromosomes, it is also essential for alignment of homologous chromosomes during meiotic metaphase I. In meiosis I, required to activate the spindle assembly checkpoint at unattached kinetochores to correct erroneous kinetochore-microtubule attachments. |
| Protein Name | Outer Kinetochore Knl1 Complex Subunit ZwintZw10 InteractorZw10-Interacting Protein 1Zwint-1 |
| Database Links | Reactome: R-HSA-141444Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-5663220Reactome: R-HSA-68877Reactome: R-HSA-9648025 |
| Cellular Localisation | NucleusChromosomeCentromereKinetochoreLocalizes To Kinetochores From Late Prophase To AnaphaseLocalizes To Kinetochores Both During Mitosis And Meiosis |
| Alternative Antibody Names | Anti-Outer Kinetochore Knl1 Complex Subunit Zwint antibodyAnti-Zw10 Interactor antibodyAnti-Zw10-Interacting Protein 1 antibodyAnti-Zwint-1 antibodyAnti-ZWINT antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
