• Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using ZFYVE19 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of lysates from HT-29 cells, using ZFYVE19 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using ZFYVE19 Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Anti-ZFYVE19 antibody (311-360 aa) (STJ96307)

SKU:
STJ96307

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Abscission/NoCut checkpoint regulator (311-360 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ZFYVE19
Gene ID: 84936
Uniprot ID: ANCHR_HUMAN
Immunogen Region: 311-360 aa
Specificity: ZFYVE19 Polyclonal Antibody detects endogenous levels of ZFYVE19 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human ZFYVE19 at the amino acid range 311-360
Post Translational Modifications Phosphorylated in vitro at Ser-22 by AURKB.however, phosphorylation at this site could not be confirmed in vivo.
Function Key regulator of abscission step in cytokinesis: part of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage. Together with CHMP4C, required to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis. Deactivation of AURKB results in dephosphorylation of CHMP4C followed by its dissociation from ZFYVE19/ANCHR and VPS4 and subsequent abscission.
Protein Name Abscission/Nocut Checkpoint Regulator
Anchr
Mll Partner Containing Fyve Domain
Zinc Finger Fyve Domain-Containing Protein 19
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Cleavage Furrow
Midbody
Midbody Ring
Localizes Mainly On Centrosomes In Interphase And Early Mitosis
Localizes At The Cleavage Furrow And Midbody Ring In Late Mitosis And Cytokinesis
Alternative Antibody Names Anti-Abscission/Nocut Checkpoint Regulator antibody
Anti-Anchr antibody
Anti-Mll Partner Containing Fyve Domain antibody
Anti-Zinc Finger Fyve Domain-Containing Protein 19 antibody
Anti-ZFYVE19 antibody
Anti-ANCHR antibody
Anti-MPFYVE antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance