Anti-ZDHHC5 antibody (130-230) (STJ11100085)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ZDHHC5 (130-230) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ZDHHC5 |
| Gene ID | 25921 |
| Uniprot ID | ZDHC5_HUMAN |
| Immunogen Region | 130-230 |
| Immunogen Sequence | FDHHCPWVNNCIGRRNYRYF FLFLLSLTAHIMGVFGFGLL YVLYHIEELSGVRTAVTMAV MCVAGLFFIPVAGLTGFHVV LVARGRTTNEQVTGKFRGGV N |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 130-230 of human ZDHHC5 (NP_056272.2). |
Additional Info
| Post Translational Modifications | Phosphorylation regulates association with endocytic proteins and its subcellular localization. Phosphorylation by LYN during fatty acid uptake leads to inactivation of the activity. Autopalmitoylated. Palmitoylation of the C-terminal tail regulates stimulation-dependent plasma membrane motility. |
| Function | Palmitoyltransferase that catalyzes the addition of palmitate onto various protein substrates such as CTNND2, CD36, GSDMD, NLRP3, NOD1, NOD2, STAT3 and S1PR1 thus plays a role in various biological processes including cell adhesion, inflammation, fatty acid uptake, bacterial sensing or cardiac functions. Plays an important role in the regulation of synapse efficacy by mediating palmitoylation of delta-catenin/CTNND2, thereby increasing synaptic delivery and surface stabilization of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptors (AMPARs). Under basal conditions, remains at the synaptic membrane through FYN-mediated phosphorylation that prevents association with endocytic proteins. Neuronal activity enhances the internalization and trafficking of DHHC5 from spines to dendritic shafts where it palmitoylates delta-catenin/CTNND2. Regulates cell adhesion at the plasma membrane by palmitoylating GOLGA7B and DSG2. Plays a role in innate immune response by mediating the palmitoylation of NOD1 and NOD2 and their proper recruitment to the bacterial entry site and phagosomes. Also participates in fatty acid uptake by palmitoylating CD36 and thereby targeting it to the plasma membrane. Upon binding of fatty acids to CD36, gets phosphorylated by LYN leading to inactivation and subsequent CD36 caveolar endocytosis. Controls oligodendrocyte development by catalyzing STAT3 palmitoylation. Acts as a regulator of inflammatory response by mediating palmitoylation of NLRP3 and GSDMD. Palmitoylates NLRP3 to promote inflammasome assembly and activation. Activates pyroptosis by catalyzing palmitoylation of gasdermin-D (GSDMD), thereby promoting membrane translocation and pore formation of GSDMD. |
| Protein Name | Palmitoyltransferase Zdhhc5Zinc Finger Dhhc Domain-Containing Protein 5Dhhc-5Zinc Finger Protein 375 |
| Database Links | Reactome: R-HSA-9694548 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinSynapse |
| Alternative Antibody Names | Anti-Palmitoyltransferase Zdhhc5 antibodyAnti-Zinc Finger Dhhc Domain-Containing Protein 5 antibodyAnti-Dhhc-5 antibodyAnti-Zinc Finger Protein 375 antibodyAnti-ZDHHC5 antibodyAnti-KIAA1748 antibodyAnti-ZNF375 antibody |
Information sourced from Uniprot.org