• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using XPA Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of lysates from COLO205 cells, using XPA Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using XPA Polyclonal Antibody diluted at 1：500. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-XPA antibody (211-260 aa) (STJ96279)

SKU:
STJ96279

£48.00 - £252.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DNA repair protein complementing XP-A cells (211-260 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: XPA
Gene ID: 7507
Uniprot ID: XPA_HUMAN
Immunogen Region: 211-260 aa
Specificity: XPA Polyclonal Antibody detects endogenous levels of XPA protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human XPA at the amino acid range 211-260
Post Translational Modifications ATR-dependent phosphorylation of XPA at Ser-196 is important for cell survival in response to UV damage. Ubiquitinated by HERC2 leading to degradation by the proteasome.
Function Involved in DNA nucleotide excision repair (NER). Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHEK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation. During NER stimulates the 5'-3' helicase activity of XPD/ERCC2 and the DNA translocase activity of XPB/ERCC3. Connects XPD/ERCC2 and XPB/ERCC3 during NER, retaining DNA near the XPB/ERCC3 active site, and stabilizing the complex in a different conformation than in transcribing TFIIH.
Protein Name Dna Repair Protein Complementing Xp-A Cells
Xeroderma Pigmentosum Group A-Complementing Protein
Database Links Reactome: R-HSA-5696395
Reactome: R-HSA-5696400
Reactome: R-HSA-6781823
Reactome: R-HSA-6782135
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Dna Repair Protein Complementing Xp-A Cells antibody
Anti-Xeroderma Pigmentosum Group A-Complementing Protein antibody
Anti-XPA antibody
Anti-XPAC antibody

Information sourced from Uniprot.org

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