• Immunofluorescence analysis of HeLa cells using WFS1 Rabbit polyclonal antibody (STJ26110) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates, using WFS1 antibody (STJ26110) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-WFS1 antibody (1-285) (STJ26110)

SKU:
STJ26110

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-WFS1 (1-285) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WFS1
Gene ID: 7466
Uniprot ID: WFS1_HUMAN
Immunogen Region: 1-285
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-285 of human WFS1 (NP_005996.2).
Immunogen Sequence: MDSNTAPLGPSCPQPPPAPQ PQARSRLNATASLEQERSER PRAPGPQAGPGPGVRDAAAP AEPQAQHTRSRERADGTGPT KGDMEIPFEEVLERAKAGDP KAQTEVGKHYLQLAGDTDEE LNSCTAVDWLVLAAKQGRRE AVKLLRRCLADRRGITSENE REVRQLSSETDLERAVRKAA LVMYWKLNPKKKKQVAVAEL LENVGQVNEHDGGAQPGPVP KSLQKQRRMLERLVSSESK
Tissue Specificity Highly expressed in heart followed by brain, placenta, lung and pancreas. Weakly expressed in liver, kidney and skeletal muscle. Also expressed in islet and beta-cell insulinoma cell line.
Function Participates in the regulation of cellular Ca(2+) homeostasis, at least partly, by modulating the filling state of the endoplasmic reticulum Ca(2+) store. Negatively regulates the ER stress response and positively regulates the stability of V-ATPase subunits ATP6V1A and ATP1B1 by preventing their degradation through an unknown proteasome-independent mechanism.
Protein Name Wolframin
Database Links Reactome: R-HSA-381038
Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Cytoplasmic Vesicle
Secretory Vesicle
Co-Localizes With Atp6v1a In The Secretory Granules In Neuroblastoma Cell Lines
Alternative Antibody Names Anti-Wolframin antibody
Anti-WFS1 antibody

Information sourced from Uniprot.org

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