| Host: |
Rabbit |
| Applications: |
ELISA/IHC/IP/WB |
| Reactivity: |
Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-WEE2 (60-110) is suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Purification: |
Affinity Purified |
| Concentration: |
0.5-1.5 µg/µl |
| Dilution Range: |
WB: 1:2, 500-1:5, 000ELISA: 1:50, 000IP: 1:200IHC: 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
Wee2 |
| Gene ID: |
381759 |
| Uniprot ID: |
WEE2_MOUSE |
| Immunogen Region: |
60-110 |
| Immunogen: |
Synthetic peptide taken within amino acid region 60-110 on mouse Wee1-like protein kinase isoform 2. |
| Post Translational Modifications | Phosphorylated by PKA at Ser-15 in vitro, leading to activate kinase activity. Phosphorylation at Ser-15 by CaMK2, leading to increase its activity and promote metaphase II exit during egg activation. |
| Function | Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation. |
| Protein Name | Wee1-Like Protein Kinase 2Wee1-Like Protein Kinase 1bWee1b KinaseMwee1b |
| Cellular Localisation | CytoplasmNucleusLocalizes Mainly In The NucleusExported From The Nucleus To The Cytoplasm Before Germinal Vesicle Breakdown (Gvbd)Allowing Meiosis Resumption |
| Alternative Antibody Names | Anti-Wee1-Like Protein Kinase 2 antibodyAnti-Wee1-Like Protein Kinase 1b antibodyAnti-Wee1b Kinase antibodyAnti-Mwee1b antibodyAnti-Wee2 antibodyAnti-Wee1b antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
