• Immunocytochemistry staining of HeLa cells fixed in 1% Paraformaldehyde and then permeabilized in 0.1% Triton X-100, next using WDR77 mouse mAb (dilution 1:100).
  • Western blot detection of WDR77 in C6, 3T3 and K562 cell lysates using WDR77 mouse mAb (1:1000 diluted).Predicted band size:42KDa.Observed band size:42KDa.

Anti-WDR77 antibody [8A10-C10-E8] (STJ99206)

SKU:
STJ99206

£139.00 - £336.00
Current Stock:
Host: Mouse
Applications: WB/ICC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Methylosome protein WDR77 is suitable for use in Western Blot and Immunocytochemistry research applications.
Clonality: Monoclonal
Clone ID: 8A10-C10-E8
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:1000
ICC 1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WDR77
Gene ID: 79084
Uniprot ID: MEP50_HUMAN
Specificity: This antibody detects endogenous levels of WDR77 and does not cross-react with related proteins.
Immunogen: Purified recombinant human WDR77 protein fragments expressed in E.coli.
Function Non-catalytic component of the methylosome complex, composed of PRMT5, WDR77 and CLNS1A, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The methylosome complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.
Protein Name Methylosome Protein Wdr77
Androgen Receptor Cofactor P44
Methylosome Protein 50
Mep-50
Wd Repeat-Containing Protein 77
P44/Mep50
Database Links Reactome: R-HSA-191859
Reactome: R-HSA-3214858
Cellular Localisation Nucleus
Cytoplasm
Nuclear In Leydig Cells And Cytoplasmic In Germ Cells During Fetal Testicular Development
In Adult Testis
Predominantly Nuclear
Subcellular Location Varies From Nuclear To Cytoplasmic In Various Tumors
Alternative Antibody Names Anti-Methylosome Protein Wdr77 antibody
Anti-Androgen Receptor Cofactor P44 antibody
Anti-Methylosome Protein 50 antibody
Anti-Mep-50 antibody
Anti-Wd Repeat-Containing Protein 77 antibody
Anti-P44/Mep50 antibody
Anti-WDR77 antibody
Anti-MEP50 antibody
Anti-WD45 antibody
Anti-HKMT1069 antibody
Anti-Nbla10071 antibody

Information sourced from Uniprot.org

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