Anti-WDR77 antibody [8A10-C10-E8] (STJ99206)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenPurified recombinant human WDR77 protein fragments expressed in E.coli.
General Information
| Short Description | Mouse monoclonal anti-Methylosome protein WDR77 for use in WB and ICC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ICC |
| Host | Mouse |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 8A10-C10-E8 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:1000ICC 1:100 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | WDR77 |
| Gene ID | 79084 |
| Uniprot ID | MEP50_HUMAN |
| Immunogen | Purified recombinant human WDR77 protein fragments expressed in E.coli. |
| Specificity | This antibody detects endogenous levels of WDR77 and does not cross-react with related proteins. |
Additional Info
| Function | Non-catalytic component of the methylosome complex, composed of PRMT5, WDR77 and CLNS1A, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The methylosome complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. |
| Protein Name | Methylosome Protein Wdr77Androgen Receptor Cofactor P44Methylosome Protein 50Mep-50Wd Repeat-Containing Protein 77P44/Mep50 |
| Database Links | Reactome: R-HSA-191859Reactome: R-HSA-3214858 |
| Cellular Localisation | NucleusCytoplasmNuclear In Leydig Cells And Cytoplasmic In Germ Cells During Fetal Testicular DevelopmentIn Adult TestisPredominantly NuclearSubcellular Location Varies From Nuclear To Cytoplasmic In Various Tumors |
| Alternative Antibody Names | Anti-Methylosome Protein Wdr77 antibodyAnti-Androgen Receptor Cofactor P44 antibodyAnti-Methylosome Protein 50 antibodyAnti-Mep-50 antibodyAnti-Wd Repeat-Containing Protein 77 antibodyAnti-P44/Mep50 antibodyAnti-WDR77 antibodyAnti-MEP50 antibodyAnti-WD45 antibodyAnti-HKMT1069 antibodyAnti-Nbla10071 antibody |
Information sourced from Uniprot.org