Anti-VP33B antibody (C-Term) (STJ13100465)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from the c-terminal of rat VP33B conjugated to blue carrier protein was used as the antigen. The antigen shares 91% identity with mouse and human sequences.
General Information
| Short Description | Rabbit polyclonal anti-VP33B (C-Term) for use in IHC and WB in Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB. A dilution of 1: 200 to 1: 1000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | Vps33b |
| Gene ID | 64060 |
| Uniprot ID | VP33B_RAT |
| Immunogen | A synthetic peptide from the c-terminal of rat VP33B conjugated to blue carrier protein was used as the antigen. The antigen shares 91% identity with mouse and human sequences. |
| Immunogen Region | C-Term |
| Specificity | Specific for VP33B. |
Additional Info
| Post Translational Modifications | Phosphorylated on tyrosine residues. |
| Function | May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. Mediates phagolysosomal fusion in macrophages. Proposed to be involved in endosomal maturation implicating VIPAS39. In epithelial cells, the VPS33B:VIPAS39 complex may play a role in the apical recycling pathway and in the maintenance of the apical-basolateral polarity. Seems to be involved in the sorting of specific cargos from the trans-Golgi network to alpha-granule-destined multivesicular bodies (MVBs) promoting MVBs maturation in megakaryocytes. |
| Protein Name | Vacuolar Protein Sorting-Associated Protein 33bR-Vps33b |
| Database Links | |
| Cellular Localisation | Late Endosome MembranePeripheral Membrane ProteinCytoplasmic SideLysosome MembraneEarly EndosomeCytoplasmic VesicleClathrin-Coated VesicleRecycling EndosomeColocalizes In Clusters With Vipas39 At Cytoplasmic OrganellesColocalizes With Rab11a And Vipas39 On Recycling EndosomesColocalizes With Ap-3ClathrinRab5 And Rab7b |
| Alternative Antibody Names | Anti-Vacuolar Protein Sorting-Associated Protein 33b antibodyAnti-R-Vps33b antibodyAnti-Vps33b antibody |
Information sourced from Uniprot.org