| Host: |
Rabbit |
| Applications: |
IHC/WB |
| Reactivity: |
Rat/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-VP33B (C-Term) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Lyophilised |
| Purification: |
IgG purified |
| Dilution Range: |
IHC, WB. A dilution of 1: 200 to 1: 1000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
Vps33b |
| Gene ID: |
64060 |
| Uniprot ID: |
VP33B_RAT |
| Immunogen Region: |
C-Term |
| Specificity: |
Specific for VP33B. |
| Immunogen: |
A synthetic peptide from the c-terminal of rat VP33B conjugated to blue carrier protein was used as the antigen. The antigen shares 91% identity with mouse and human sequences. |
| Post Translational Modifications | Phosphorylated on tyrosine residues. |
| Function | May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. Mediates phagolysosomal fusion in macrophages. Proposed to be involved in endosomal maturation implicating VIPAS39. In epithelial cells, the VPS33B:VIPAS39 complex may play a role in the apical recycling pathway and in the maintenance of the apical-basolateral polarity. Seems to be involved in the sorting of specific cargos from the trans-Golgi network to alpha-granule-destined multivesicular bodies (MVBs) promoting MVBs maturation in megakaryocytes. |
| Protein Name | Vacuolar Protein Sorting-Associated Protein 33bR-Vps33b |
| Cellular Localisation | Late Endosome MembranePeripheral Membrane ProteinCytoplasmic SideLysosome MembraneEarly EndosomeCytoplasmic VesicleClathrin-Coated VesicleRecycling EndosomeColocalizes In Clusters With Vipas39 At Cytoplasmic OrganellesColocalizes With Rab11a And Vipas39 On Recycling EndosomesColocalizes With Ap-3ClathrinRab5 And Rab7b |
| Alternative Antibody Names | Anti-Vacuolar Protein Sorting-Associated Protein 33b antibodyAnti-R-Vps33b antibodyAnti-Vps33b antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
