• Confocal imaging of HeLa cells using Vinculin Rabbit monoclonal antibody (STJ11101832, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 60x.
  • Western blot analysis of various lysates, using Vinculin Rabbit monoclonal antibody (STJ11101832) at 1:250000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-VCL antibody (800-900) [S2MR] (STJ11101832)

SKU:
STJ11101832

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Vinculin (800-900) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:10000-1:300000
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VCL
Gene ID: 7414
Uniprot ID: VINC_HUMAN
Immunogen Region: 800-900
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 800-900 of human Vinculin (NP_054706.1).
Immunogen Sequence: DAKAVAGNISDPGLQKSFLD SGYRILGAVAKVREAFQPQE PDFPPPPPDLEQLRLTDELA PPKPPLPEGEVPPPRPPPPE EKDEEFPEQKAGEVINQPMM M
Tissue Specificity Metavinculin is muscle-specific.
Post Translational Modifications Phosphorylated.on serines, threonines and tyrosines. Phosphorylation on Tyr-1133 in activated platelets affects head-tail interactions and cell spreading but has no effect on actin binding nor on localization to focal adhesion plaques. Acetylated.mainly by myristic acid but also by a small amount of palmitic acid.
Function Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.
Protein Name Vinculin
Metavinculin
Mv
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-445355
Reactome: R-HSA-5674135
Reactome: R-HSA-6798695
Reactome: R-HSA-6802946
Reactome: R-HSA-6802948
Reactome: R-HSA-6802952
Reactome: R-HSA-6802955
Reactome: R-HSA-9649948
Reactome: R-HSA-9656223
Reactome: R-HSA-9725370
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cell Junction
Adherens Junction
Focal Adhesion
Cytoplasm
Cytoskeleton
Sarcolemma
Cell Projection
Podosome
Recruitment To Cell-Cell Junctions Occurs In A Myosin Ii-Dependent Manner
Interaction With Ctnnb1 Is Necessary For Its Localization To The Cell-Cell Junctions
Alternative Antibody Names Anti-Vinculin antibody
Anti-Metavinculin antibody
Anti-Mv antibody
Anti-VCL antibody

Information sourced from Uniprot.org

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