Anti-VCL antibody (800-900) [S2MR] (STJ11101832)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal Vinculin (800-900) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S2MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:300000-1:1280000IF/ICC:1:800-1:3200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | VCL |
| Gene ID | 7414 |
| Uniprot ID | VINC_HUMAN |
| Immunogen Region | 800-900 |
| Immunogen Sequence | DAKAVAGNISDPGLQKSFLD SGYRILGAVAKVREAFQPQE PDFPPPPPDLEQLRLTDELA PPKPPLPEGEVPPPRPPPPE EKDEEFPEQKAGEVINQPMM M |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 800-900 of human Vinculin (NP_054706.1). |
Additional Info
| Tissue Specificity | Metavinculin is muscle-specific. |
| Post Translational Modifications | Phosphorylated.on serines, threonines and tyrosines. Phosphorylation on Tyr-1133 in activated platelets affects head-tail interactions and cell spreading but has no effect on actin binding nor on localization to focal adhesion plaques. Acetylated.mainly by myristic acid but also by a small amount of palmitic acid. |
| Function | Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. |
| Protein Name | VinculinMetavinculinMv |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-445355Reactome: R-HSA-5674135Reactome: R-HSA-6798695Reactome: R-HSA-6802946Reactome: R-HSA-6802948Reactome: R-HSA-6802952Reactome: R-HSA-6802955Reactome: R-HSA-9649948Reactome: R-HSA-9656223Reactome: R-HSA-9725370Reactome: R-HSA-9856530Reactome: R-HSA-9860927 |
| Cellular Localisation | Cell MembranePeripheral Membrane ProteinCytoplasmic SideCell JunctionAdherens JunctionFocal AdhesionCytoplasmCytoskeletonSarcolemmaCell ProjectionPodosomeRecruitment To Cell-Cell Junctions Occurs In A Myosin Ii-Dependent MannerInteraction With Ctnnb1 Is Necessary For Its Localization To The Cell-Cell Junctions |
| Alternative Antibody Names | Anti-Vinculin antibodyAnti-Metavinculin antibodyAnti-Mv antibodyAnti-VCL antibody |
Information sourced from Uniprot.org