![Anti-VIM antibody (367-466) [S7MR] (STJ11101727)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90642/360564/STJ11101727_1__01580.1713125242.jpg?c=1 "Anti-VIM antibody (367-466) [S7MR] (STJ11101727)")
![Immunoprecipitation analysis of 300 Mu g extracts of Jurkat cells using 3 Mu g [KD Validated] Vimentin Rabbit monoclonal antibody (STJ11101727). Western blot was performed from the immunoprecipitate using Vimentin antibody (STJ11101727) at a dilution of 1:1000.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90642/360565/STJ11101727_2__78064.1713125242.jpg?c=1 "Immunoprecipitation analysis of 300 Mu g extracts of Jurkat cells using 3 Mu g [KD Validated] Vimentin Rabbit monoclonal antibody (STJ11101727). Western blot was performed from the immunoprecipitate using Vimentin antibody (STJ11101727) at a dilution of 1:1000.")
![Confocal imaging of paraffin-embedded Rat testis tissue using [KD Validated] Vimentin Rabbit monoclonal antibody (STJ11101727, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining. Objective: 40x.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90642/360566/STJ11101727_3__77864.1713125243.jpg?c=1 "Confocal imaging of paraffin-embedded Rat testis tissue using [KD Validated] Vimentin Rabbit monoclonal antibody (STJ11101727, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining. Objective: 40x.")
![Confocal imaging of HeLa cells usingย [KD Validated] Vimentin Rabbit monoclonal antibody (STJ11101727, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABfloยฎ 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90642/360567/STJ11101727_4__04683.1713125244.jpg?c=1 "Confocal imaging of HeLa cells usingย [KD Validated] Vimentin Rabbit monoclonal antibody (STJ11101727, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABfloยฎ 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.")
![Confocal imaging ofย paraffin-embedded Rat kidney tissue usingย [KD Validated] Vimentin Rabbit monoclonal antibody (STJ11101727, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining. Objective: 40x.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90642/360568/STJ11101727_5__91416.1713125245.jpg?c=1 "Confocal imaging ofย paraffin-embedded Rat kidney tissue usingย [KD Validated] Vimentin Rabbit monoclonal antibody (STJ11101727, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining. Objective: 40x.")
![Confocal imaging of C6 cells usingย [KD Validated] Vimentin Rabbit monoclonal antibody (STJ11101727, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABfloยฎ 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90642/360569/STJ11101727_6__99205.1713125246.jpg?c=1 "Confocal imaging of C6 cells usingย [KD Validated] Vimentin Rabbit monoclonal antibody (STJ11101727, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABfloยฎ 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.")
![Confocal imaging of C2C12 cells usingย [KD Validated] Vimentin Rabbit monoclonal antibody (STJ11101727, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABfloยฎ 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90642/360570/STJ11101727_7__02099.1713125246.jpg?c=1 "Confocal imaging of C2C12 cells usingย [KD Validated] Vimentin Rabbit monoclonal antibody (STJ11101727, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABfloยฎ 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.")
![Immunohistochemistry analysis of Vimentin in paraffin-embedded human lung cancer using [KD Validated] Vimentin Rabbit monoclonal antibody (STJ11101727) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90642/360571/STJ11101727_8__72564.1713125247.jpg?c=1 "Immunohistochemistry analysis of Vimentin in paraffin-embedded human lung cancer using [KD Validated] Vimentin Rabbit monoclonal antibody (STJ11101727) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.")
![Immunohistochemistry analysis of Vimentin in paraffin-embedded human liver cancer using [KD Validated] Vimentin Rabbit monoclonal antibody (STJ11101727) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90642/360572/STJ11101727_9__91853.1713125248.jpg?c=1 "Immunohistochemistry analysis of Vimentin in paraffin-embedded human liver cancer using [KD Validated] Vimentin Rabbit monoclonal antibody (STJ11101727) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.")
![Immunohistochemistry analysis of Vimentin in paraffin-embedded human colon using [KD Validated] Vimentin Rabbit monoclonal antibody (STJ11101727) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90642/360573/STJ11101727_10__77584.1713125248.jpg?c=1 "Immunohistochemistry analysis of Vimentin in paraffin-embedded human colon using [KD Validated] Vimentin Rabbit monoclonal antibody (STJ11101727) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.")
![Western blot analysis of lysates from wild type (WT) and Vimentin knockdown (KD) 293T cells, using [KD Validated] Vimentin Rabbit monoclonal antibody (STJ11101727) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90642/360574/STJ11101727_11__93562.1713125249.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Vimentin knockdown (KD) 293T cells, using [KD Validated] Vimentin Rabbit monoclonal antibody (STJ11101727) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of various lysates using [KD Validated] Vimentin Rabbit monoclonal antibody (STJ11101727) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90642/360575/STJ11101727_12__80538.1713125250.jpg?c=1 "Western blot analysis of various lysates using [KD Validated] Vimentin Rabbit monoclonal antibody (STJ11101727) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Anti-VIM antibody (367-466) [S7MR] (STJ11101727)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/90642/360564/STJ11101727_1__01580.1713125242.jpg?c=1 "Anti-VIM antibody (367-466) [S7MR] (STJ11101727)")
Anti-VIM antibody (367-466) [S7MR] (STJ11101727)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal Vimentin (367-466) antibody for use in WB, IHC-P, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S7MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:20000-1:120000IHC-P:1:1000-1:4000IF/ICC:1:200-1:2000IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on yo |
| Formulation | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | VIM |
| Gene ID | 7431 |
| Uniprot ID | VIME_HUMAN |
| Immunogen Region | 367-466 |
| Immunogen Sequence | DEIQNMKEEMARHLREYQDL LNVKMALDIEIATYRKLLEG EESRISLPLPNFSSLNLRET NLDSLPLVDTHSKRTLLIKT VETRDGQVINETSQHHDDLE |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 367-466 of human Vimentin (P08670). |
Additional Info
| Tissue Specificity | Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. |
| Post Translational Modifications | Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin. One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33. Phosphorylated on tyrosine residues by SRMS. O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status. S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. |
| Function | Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front. Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. |
| Protein Name | Vimentin |
| Database Links | Reactome: R-HSA-264870Reactome: R-HSA-390522Reactome: R-HSA-6785807Reactome: R-HSA-9013422Reactome: R-HSA-9613829Reactome: R-HSA-9615710Reactome: R-HSA-9646399 |
| Cellular Localisation | CytoplasmCytoskeletonNucleus MatrixCell Membrane |
| Alternative Antibody Names | Anti-Vimentin antibodyAnti-VIM antibody |
Information sourced from Uniprot.org