• Immunofluorescence analysis of NIH/3T3 cells using VEGFA Rabbit polyclonal antibody (STJ114190) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates, using VEGFA Rabbit polyclonal antibody (STJ114190) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-VEGFA antibody (27-126) (STJ114190)

SKU:
STJ114190

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-VEGF (27-126) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VEGFA
Gene ID: 7422
Uniprot ID: VEGFA_HUMAN
Immunogen Region: 27-126
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 27-126AA of human VEGFA (NP_001165099.1).
Immunogen Sequence: APMAEGGGQNHHEVVKFMDV YQRSYCHPIETLVDIFQEYP DEIEYIFKPSCVPLMRCGGC CNDEGLECVPTEESNITMQI MRIKPHQGQHIGEMSFLQHN
Tissue Specificity Higher expression in pituitary tumors than the pituitary gland. Isoform VEGF189: Widely expressed. Isoform VEGF165: Widely expressed. Isoform VEGF121: Widely expressed. Isoform VEGF206: Not widely expressed. Isoform VEGF145: Not widely expressed.
Post Translational Modifications Vascular endothelial growth factor A, long form: Produced by use of an alternative upstream CUG codon and post-translationally processed into the N-terminal N-VEGF form and the C-terminal secreted VEGFA form.
Function N-VEGF: Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A. Involved in protecting cells from hypoxia-mediated cell death. VEGFA: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development. Also binds the DEAR/FBXW7-AS1 receptor. Isoform VEGF165B: Binds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
Protein Name Vascular Endothelial Growth Factor A - Long Form
L-Vegf
Vascular Permeability Factor
Vpf Cleaved Into - N-Vegf - Vegfa
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-1234158
Reactome: R-HSA-194138
Reactome: R-HSA-194313
Reactome: R-HSA-195399
Reactome: R-HSA-4420097 P15692-4
Reactome: R-HSA-5218921 P15692-4
Reactome: R-HSA-6785807
Reactome: R-HSA-8866910
Reactome: R-HSA-9679191 P15692-4
Cellular Localisation N-Vegf: Cytoplasm
Nucleus
Cytoplasmic In Normoxic Conditions And Localizes To The Nucleus Under Hypoxic Conditions
Vegfa: Secreted
Isoform L-Vegf189: Endoplasmic Reticulum
Golgi Apparatus
Secreted
Extracellular Space
Extracellular Matrix
Isoform Vegf121: Secreted
Isoform Vegf165: Secreted
Isoform Vegf189: Secreted
Cell-Associated After Secretion And Is Bound Avidly By Heparin And The Extracellular Matrix
Although It May Be Released As A Soluble Form By Heparin
Heparinase Or Plasmin
Alternative Antibody Names Anti-Vascular Endothelial Growth Factor A - Long Form antibody
Anti-L-Vegf antibody
Anti-Vascular Permeability Factor antibody
Anti-Vpf Cleaved Into - N-Vegf - Vegfa antibody
Anti-VEGFA antibody
Anti-VEGF antibody

Information sourced from Uniprot.org

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