Anti-VAMP-1/2/3 antibody (21-70 aa) (STJ96215)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human VAMP-1/2/3 at the amino acid range 21-70
General Information
| Short Description | Rabbit polyclonal anti-Vesicle-associated membrane protein 1 and Vesicle-associated membrane protein 2 and Vesicle-associated membrane protein 3 (21-70 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilu |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000IHC 1:100-1:300ELISA 1:10000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | VAMP2VAMP3684493416843VAMP2_HUMANVAMP3_HUMANVAMP1_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human VAMP-1/2/3 at the amino acid range 21-70 |
| Immunogen Region | 21-70 aa |
| Specificity | VAMP-1/2/3 Polyclonal Antibody detects endogenous levels of VAMP-1/2/3 protein. |
Additional Info
| Post Translational Modifications | (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type B (BoNT/B, botB) which probably hydrolyzes the 78-Gln-|-Phe-79 bond and inhibits neurotransmitter release. (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type D (BoNT/D, botD) which probably hydrolyzes the 61-Arg-|-Leu-62 bond and inhibits neurotransmitter release. BoNT/D has low catalytic activity on this protein due to its sequence. Note that humans are not known to be infected by C.botulinum type D. (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type F (BoNT/F, botF) which probably hydrolyzes the 60-Gln-|-Lys-61 bond and inhibits neurotransmitter release. (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type X (BoNT/X) which probably hydrolyzes the 68-Arg-|-Ala-69 bond and inhibits neurotransmitter release. It remains unknown whether BoNT/X is ever produced, or what organisms it targets. |
| Function | Involved in the targeting and/or fusion of transport vesicles to their target membrane. |
| Protein Name | Vesicle-Associated Membrane Protein 1Vamp-1Synaptobrevin-1 |
| Database Links | Reactome: R-HSA-5250955Reactome: R-HSA-5250981Reactome: R-HSA-5250989 |
| Cellular Localisation | Isoform 1: Cytoplasmic VesicleSecretory VesicleSynaptic Vesicle MembraneSingle-Pass Type Iv Membrane ProteinCell JunctionSynapseSynaptosomeIsoform 2: Cytoplasmic Vesicle MembraneIsoform 3: Mitochondrion Outer Membrane |
| Alternative Antibody Names | Anti-Vesicle-Associated Membrane Protein 1 antibodyAnti-Vamp-1 antibodyAnti-Synaptobrevin-1 antibodyAnti-VAMP1 antibodyAnti-SYB1 antibody |
Information sourced from Uniprot.org