| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-USP30 (57-390) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
USP30 |
| Gene ID: |
84749 |
| Uniprot ID: |
UBP30_HUMAN |
| Immunogen Region: |
57-390 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 57-390 of human USP30 (NP_116052.2). |
| Immunogen Sequence: |
TERKKRRKGLVPGLVNLGNT CFMNSLLQGLSACPAFIRWL EEFTSQYSRDQKEPPSHQYL SLTLLHLLKALSCQEVTDDE VLDASCLLDVLRMYRWQISS FEEQDAHELFHVITSSLEDE RDRQPRVTHLFDVHSLEQQS EITPKQITCRTRGSPHPTSN HWKSQHPFHGRLTSNMVCKH CEHQSPVRFDTFDSLSLSIP AATWGHPLTLDHCLHHFISS ESVRDVVCDNCTKIEAKGT |
| Tissue Specificity | Expressed in skeletal muscle, pancreas, liver and kidney. |
| Post Translational Modifications | Ubiquitinated by parkin (PRKN) at Lys-235 and Lys-289, leading to its degradation. |
| Function | Deubiquitinating enzyme tethered to the mitochondrial outer membrane that acts as a key inhibitor of mitophagy by counteracting the action of parkin (PRKN): hydrolyzes ubiquitin attached by parkin on target proteins, such as RHOT1/MIRO1 and TOMM20, thereby blocking parkin's ability to drive mitophagy. Preferentially cleaves 'Lys-6'- and 'Lys-11'-linked polyubiquitin chains, 2 types of linkage that participate in mitophagic signaling. Does not cleave efficiently polyubiquitin phosphorylated at 'Ser-65'. Acts as negative regulator of mitochondrial fusion by mediating deubiquitination of MFN1 and MFN2. |
| Protein Name | Ubiquitin Carboxyl-Terminal Hydrolase 30Deubiquitinating Enzyme 30Ubiquitin Thioesterase 30Ubiquitin-Specific-Processing Protease 30Ub-Specific Protease 30 |
| Database Links | Reactome: R-HSA-5689880Reactome: R-HSA-9664873 |
| Cellular Localisation | Mitochondrion Outer Membrane |
| Alternative Antibody Names | Anti-Ubiquitin Carboxyl-Terminal Hydrolase 30 antibodyAnti-Deubiquitinating Enzyme 30 antibodyAnti-Ubiquitin Thioesterase 30 antibodyAnti-Ubiquitin-Specific-Processing Protease 30 antibodyAnti-Ub-Specific Protease 30 antibodyAnti-USP30 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
