• Western blot analysis of lysates from HeLa cells, using USP30 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using USP30 Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Anti-USP30 antibody (31-80 aa) (STJ96199)

SKU:
STJ96199

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Ubiquitin carboxyl-terminal hydrolase 30 (31-80 aa) is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: USP30
Gene ID: 84749
Uniprot ID: UBP30_HUMAN
Immunogen Region: 31-80 aa
Specificity: USP30 Polyclonal Antibody detects endogenous levels of USP30 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human USP30 at the amino acid range 31-80
Post Translational Modifications Ubiquitinated by parkin (PRKN) at Lys-235 and Lys-289, leading to its degradation.
Function Deubiquitinating enzyme tethered to the mitochondrial outer membrane that acts as a key inhibitor of mitophagy by counteracting the action of parkin (PRKN): hydrolyzes ubiquitin attached by parkin on target proteins, such as RHOT1/MIRO1 and TOMM20, thereby blocking parkin's ability to drive mitophagy. Preferentially cleaves 'Lys-6'- and 'Lys-11'-linked polyubiquitin chains, 2 types of linkage that participate in mitophagic signaling. Does not cleave efficiently polyubiquitin phosphorylated at 'Ser-65'. Acts as negative regulator of mitochondrial fusion by mediating deubiquitination of MFN1 and MFN2.
Protein Name Ubiquitin Carboxyl-Terminal Hydrolase 30
Deubiquitinating Enzyme 30
Ubiquitin Thioesterase 30
Ubiquitin-Specific-Processing Protease 30
Ub-Specific Protease 30
Database Links Reactome: R-HSA-5689880
Reactome: R-HSA-9664873
Cellular Localisation Mitochondrion Outer Membrane
Alternative Antibody Names Anti-Ubiquitin Carboxyl-Terminal Hydrolase 30 antibody
Anti-Deubiquitinating Enzyme 30 antibody
Anti-Ubiquitin Thioesterase 30 antibody
Anti-Ubiquitin-Specific-Processing Protease 30 antibody
Anti-Ub-Specific Protease 30 antibody
Anti-USP30 antibody

Information sourced from Uniprot.org

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