• Immunofluorescence analysis of NIH-3T3 cells using USP30 Rabbit polyclonal antibody (STJ114728) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using USP30 Rabbit polyclonal antibody (STJ114728) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-USP30 antibody (57-390) (STJ114728)

SKU:
STJ114728

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-USP30 (57-390) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: USP30
Gene ID: 84749
Uniprot ID: UBP30_HUMAN
Immunogen Region: 57-390
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 57-390 of human USP30 (NP_116052.2).
Immunogen Sequence: TERKKRRKGLVPGLVNLGNT CFMNSLLQGLSACPAFIRWL EEFTSQYSRDQKEPPSHQYL SLTLLHLLKALSCQEVTDDE VLDASCLLDVLRMYRWQISS FEEQDAHELFHVITSSLEDE RDRQPRVTHLFDVHSLEQQS EITPKQITCRTRGSPHPTSN HWKSQHPFHGRLTSNMVCKH CEHQSPVRFDTFDSLSLSIP AATWGHPLTLDHCLHHFISS ESVRDVVCDNCTKIEAKGT
Tissue Specificity Expressed in skeletal muscle, pancreas, liver and kidney.
Post Translational Modifications Ubiquitinated by parkin (PRKN) at Lys-235 and Lys-289, leading to its degradation.
Function Deubiquitinating enzyme tethered to the mitochondrial outer membrane that acts as a key inhibitor of mitophagy by counteracting the action of parkin (PRKN): hydrolyzes ubiquitin attached by parkin on target proteins, such as RHOT1/MIRO1 and TOMM20, thereby blocking parkin's ability to drive mitophagy. Preferentially cleaves 'Lys-6'- and 'Lys-11'-linked polyubiquitin chains, 2 types of linkage that participate in mitophagic signaling. Does not cleave efficiently polyubiquitin phosphorylated at 'Ser-65'. Acts as negative regulator of mitochondrial fusion by mediating deubiquitination of MFN1 and MFN2.
Protein Name Ubiquitin Carboxyl-Terminal Hydrolase 30
Deubiquitinating Enzyme 30
Ubiquitin Thioesterase 30
Ubiquitin-Specific-Processing Protease 30
Ub-Specific Protease 30
Database Links Reactome: R-HSA-5689880
Reactome: R-HSA-9664873
Cellular Localisation Mitochondrion Outer Membrane
Alternative Antibody Names Anti-Ubiquitin Carboxyl-Terminal Hydrolase 30 antibody
Anti-Deubiquitinating Enzyme 30 antibody
Anti-Ubiquitin Thioesterase 30 antibody
Anti-Ubiquitin-Specific-Processing Protease 30 antibody
Anti-Ub-Specific Protease 30 antibody
Anti-USP30 antibody

Information sourced from Uniprot.org

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